H00007535-M01

antibody from Abnova Corporation

Targeting: ZAP70 SRK, STD, ZAP-70

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00007535-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00007535-M01, RRID:AB_425745

Product name

ZAP70 monoclonal antibody (M01), clone 2F6

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant ZAP70.

Antigen sequence

MRLGPRWKGEALEQAIISQAPQVEKLIATTAHERM
PWYHSSLTREEAERKLYSGAQTDGKFLLRPRKEQG
TYALSLIYGKTVYHYLISQDKAGKYCIPEGTKFDT
LWQLVEYLKLKADGLIYCLKEACPNSSASNASGAA
APTLPAHPSTLTHPQRRIDTLNSDGYTPEPARITS
PDKPRPMPMDTSVYESPYSDPEELKDKKLFLKRDN
LLIADIELGCGNFGSVRQGVYRMRKKQIDVAIKVL
KQGTEKADTEEMMREAQIMHQLDNPYIVRLIGVCQ
AEALMLVMEMAGGGPLHKFLVGKREEIPVSNVAEL
LHQVSMGMKYLEEKNFVHRDLAARNVLLVNRHYAK
ISDFGLSKALGADDSYYTARSAGKWPLKWYAPECI
NFRKFSSRSDVWSYGVTMWEALSYGQKPYKKMKGP
EVMAFIEQGKRMECPPECPPELYALMSDCWIYKWE
DRPDFLTVEQRMRACYYSLASKVEGPPGSTQKAEA
ACA


Isotype

IgG

Antibody clone number

2F6

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged ZAP70 is approximately 0.3ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol