PA5-117027

antibody from Invitrogen Antibodies

Targeting: FADD GIG3, MORT1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-117027

Provider

Invitrogen Antibodies

Product name

FADD Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive Samples: 293T, MCF7, HeLa, Mouse liver, Mouse kidney, Mouse heart

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

2.34 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of FADD using FADD Polyclonal Antibody (Product # PA5-117027) at a 1:1,000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of FADD in rat kidney tissue using FADD Polyclonal Antibody (Product # PA5-117027) at a dilution of 1:200.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation analysis of FADD in 200 µg extracts of HeLa cells using FADD Polyclonal Antibody (Product # PA5-117027). Western blot was performed from the immunoprecipitate using the same antibody at a dilution of 1:100