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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
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- Product number
- NBP2-20352 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal SHC1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SHC1 Antibody [NBP2-20352] - Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SHC1 antibody [N3C2], Internal. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SHC1 Antibody [NBP2-20352] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7. 5% SDS PAGE gel, diluted at 1:3000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SHC1 Antibody [NBP2-20352] - Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with SHC1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
