Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- AP32477PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Product name
- anti SHC1 / SHC pTyr317
- Antibody type
- Polyclonal
- Antigen
- KLH-conjugated, synthetic peptide (FDDPS[pY]VN-C) corresponding to amino acids 312-319 of Human p52 SHC, containing a C-terminal cysteine. This motif is the Grb2 binding site.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.2 ml
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Supportive validation
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- Acris Antibodies GmbH (provider)
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- Experimental details
- Immunoblot Analysis (Representative blot from previous lot).Non-stimulated (lane 1) or EGF-stimulated (lane 2) HFF cell lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-SHC (Y317), 1/1000 dilution. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrows indicates phosphorylated SHC.