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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PA5-36727 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-SHC (Tyr427) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody detects endogenous protein at a molecular weight of 46, 52 and 66 kDa. Purity is >95% by SDS-PAGE.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-SHC (Tyr427) in Lane 1: HEK293T cell lysate treated with EGF (0.1 ng/mL, 30 mins), Lane 2: Raw264.7 cell lysate treated with EGF (0.1 ng/mL, 30 mins), Lane 3: PC12 cell lysate treated with EGF (0.1 ng/mL, 30 mins). Samples were incubated with Phospho-SHC (Tyr427) polyclonal antibody (Product # PA5-36727) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-SHC pTyr427 using Phospho-SHC pTyr427 polyclonal antibody (Product # PA5-36727) at a dilution of 1:500. Lane 1: HEK293T cell lysate treated with EGF (0.1 ng/mL, 30 min), Lane 2: Raw264.7 cell lysate treated with EGF (0.1 ng/mL, 30 min), Lane 3: PC12 cell lysate treated with EGF (0.1 ng/mL, 30 min).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Phospho-SHC (Tyr427) in paraffin-embedded human colon cancer tissue. Samples were incubated with Phospho-SHC (Tyr427) polyclonal antibody (Product # PA5-36727).
