PA5-36727

antibody from Invitrogen Antibodies

Targeting: SHC1 p66, SHC, ShcA

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-36727

Provider

Invitrogen Antibodies

Product name

Phospho-SHC (Tyr427) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody detects endogenous protein at a molecular weight of 46, 52 and 66 kDa.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Phospho-SHC (Tyr427) in Lane 1: HEK293T cell lysate treated with EGF (0.1 ng/mL, 30 mins), Lane 2: Raw264.7 cell lysate treated with EGF (0.1 ng/mL, 30 mins), Lane 3: PC12 cell lysate treated with EGF (0.1 ng/mL, 30 mins). Samples were incubated with Phospho-SHC (Tyr427) polyclonal antibody (Product # PA5-36727) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Phospho-SHC pTyr427 using Phospho-SHC pTyr427 polyclonal antibody (Product # PA5-36727) at a dilution of 1:500. Lane 1: HEK293T cell lysate treated with EGF (0.1 ng/mL, 30 min), Lane 2: Raw264.7 cell lysate treated with EGF (0.1 ng/mL, 30 min), Lane 3: PC12 cell lysate treated with EGF (0.1 ng/mL, 30 min).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Phospho-SHC (Tyr427) in paraffin-embedded human colon cancer tissue. Samples were incubated with Phospho-SHC (Tyr427) polyclonal antibody (Product # PA5-36727).