MAB278-100

antibody from R&D Systems

Targeting: CCL5 D17S136E, MGC17164, RANTES, SCYA5, SISd, TCP228

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB278-100

Provider

R&D Systems

Product name

Human CCL5/RANTES Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from ascites. Detects human CCL5/RANTES in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with other chemokines, including recombinant human CCL2, 3, 4, recombinant mouse (rm) CCL3, and rmCCL4.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

21445

Vial size

100 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Pathological study of archival lung tissues from five fatal cases of avian H5N1 influenza in Vietnam.

Nakajima N, Van Tin N, Sato Y, Thach HN, Katano H, Diep PH, Kumasaka T, Thuy NT, Hasegawa H, San LT, Kawachi S, Liem NT, Suzuki K, Sata T
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2013 Mar;26(3):357-69

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Up-regulation of platelet activation in hemophilia A.

van Bladel ER, Roest M, de Groot PG, Schutgens RE
Haematologica 2011 Jun;96(6):888-95

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Endothelial damage from cytomegalovirus-specific host immune response can be prevented by targeted disruption of fractalkine-CX3CR1 interaction.

Bolovan-Fritts CA, Spector SA
Blood 2008 Jan 1;111(1):175-82

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Pre-eclampsia is associated with dendritic cell recruitment into the uterine decidua.

Huang SJ, Chen CP, Schatz F, Rahman M, Abrahams VM, Lockwood CJ
The Journal of pathology 2008 Feb;214(3):328-36

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Chemokine and chemokine receptor expression in paired peripheral blood mononuclear cells and synovial tissue of patients with rheumatoid arthritis, osteoarthritis, and reactive arthritis.

Haringman JJ, Smeets TJ, Reinders-Blankert P, Tak PP
Annals of the rheumatic diseases 2006 Mar;65(3):294-300

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Rapid chemokine secretion from endothelial cells originates from 2 distinct compartments.

Øynebråten I, Bakke O, Brandtzaeg P, Johansen FE, Haraldsen G
Blood 2004 Jul 15;104(2):314-20

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

CCL5/RANTES in Mouse Small Intestine. CCL5/RANTES was detected in perfusion fixed frozen sections of mouse small intestine using Mouse Anti-Human CCL5/RANTES Monoclonal Antibody (Catalog # MAB278) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Blocking/Neutralizing

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Chemotaxis Induced by CCL5/RANTES and Neutralization by Human CCL5/RANTES Antibody. Recombinant Human CCL5/RANTES (Catalog # 278-RN) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL5/RANTES (0.01 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL5/RANTES Monoclonal Antibody (Catalog # MAB278). The ND₅₀ is typically 0.1-0.4 µg/mL.