MA5-13679

antibody from Invitrogen Antibodies

Targeting: ERBB2 CD340, HER-2, HER2, NEU, NGL

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-13679

Provider

Invitrogen Antibodies

Product name

ErbB2 (HER-2) Monoclonal Antibody (L26)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

MA5-13679 targets HER-2 in immunoprecipitation applications and shows reactivity with Human samples.

Antibody clone number

L26

Concentration

0.2 mg/mL

References

Submitted references

Association between growth factor heregulin1α and receptors in growth of ovarian cancer cell line with high potentiality of peritoneal dissemination.

Nishiyama H, Soeda S, Watanabe T, Fujimori K
Fukushima journal of medical science 2012;58(1):22-32

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BCL6 positively regulates AID and germinal center gene expression via repression of miR-155.

Basso K, Schneider C, Shen Q, Holmes AB, Setty M, Leslie C, Dalla-Favera R
The Journal of experimental medicine 2012 Dec 17;209(13):2455-65

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Lymphoepithelioma-like carcinoma of the ovary.

Ambrosio MR, Rocca BJ, Onorati M, Mourmouras V, Mastrogiulio MG, Crispino S, Liberatore C, Santopietro R
International journal of surgical pathology 2011 Aug;19(4):514-7

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Identification of a morphometrical parameter that predicts the response to splenectomy in patients with idiopathic thrombocytopenic purpura.

Bakkaloglu H, Dinccag A, Yanar H, Tunca F, Dogan O, Cermik H, Kucukkaya R
The Tohoku journal of experimental medicine 2006 Sep;210(1):49-55

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A chimeric multi-human epidermal growth factor receptor-2 B cell epitope peptide vaccine mediates superior antitumor responses.

Dakappagari NK, Pyles J, Parihar R, Carson WE, Young DC, Kaumaya PT
Journal of immunology (Baltimore, Md. : 1950) 2003 Apr 15;170(8):4242-53

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A chimeric multi-human epidermal growth factor receptor-2 B cell epitope peptide vaccine mediates superior antitumor responses.

Dakappagari NK, Pyles J, Parihar R, Carson WE, Young DC, Kaumaya PT
Journal of immunology (Baltimore, Md. : 1950) 2003 Apr 15;170(8):4242-53

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Identification of epitope regions recognized by tumor inhibitory and stimulatory anti-ErbB-2 monoclonal antibodies: implications for vaccine design.

Yip YL, Smith G, Koch J, Dübel S, Ward RL
Journal of immunology (Baltimore, Md. : 1950) 2001 Apr 15;166(8):5271-8

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of Her2 was performed using 70% confluent log phase SK-BR-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Her2 Mouse monoclonal Antibody (Product # MA5-13679) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree Celsius and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membranous and cytoplasmic localization. Panel f represents MDAMB-231 cells as negative controls, showing no Her2 staining. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.