NBP2-33064
antibody from Novus Biologicals
Targeting: ERBB2
CD340, HER-2, HER2, NEU, NGL
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [2]
- Flow cytometry [2]
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Validation data
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- Product number
- NBP2-33064 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal ErbB2/Her2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. This MAb is specific to c-erbB-2/HER-2 and shows minimal cross-reaction with other members of the family. C-erbB-2/HER-2 is a member of the EGFR family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. c-erbB-2/HER-2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody (HRB2/451) - Azide and BSA Free [NBP2-33064] - Formalin-paraffin breast invasive carcinoma stained with HER-2 Ab (HRB2/451).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody (HRB2/451) - Azide and BSA Free [NBP2-33064] - Formalin-paraffin breast DCIS (ductal carcinoma in situ)stained with HER-2 Ab (HRB2/451).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Cell Surface): ErbB2/Her2 Antibody (HRB2/451) - Azide and BSA Free [NBP2-33064] - A cell surface stain was performed on HeLa cells with ErbB2/Her2 Antibody (HRB2/451) antibody NBP2-33064R (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Dylight 550.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: ErbB2/Her2 Antibody (HRB2/451) - Azide and BSA Free [NBP2-33064] - Flow Cytometric Analysis of human trypsinized SK-BR3 cells. ErbB2/Her2 Antibody (HRB2/451) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).