Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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Validation data
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- Product number
- AP3781t - Provider product page
- Provider
- Abgent
- Product name
- Phospho-ERBB2(Y1196) Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 0.35 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Dot blot analysis of ERBB2 Antibody (Phospho Y1196) Phospho-specific Pab (Cat. #AP3781t) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.
- Primary Ab dilution
- 1:500
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Western blot analysis of extracts from A431 cells,untreated or treated with EGF,100ng/mlÿusing phospho ERBB2 Y1196 (left) or ERBB2 antibody(right)"
- Primary Ab dilution
- 1:4000
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Fluorescent confocal image of MCF7 cells stained with phospho-ERBB2-Y1196 antibody. MCF7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP3781t phospho-ERBB2- Y1196 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ¼g/ml, 5 min). Note the highly specific localization of the phospho-ERBB2-Y1196 to the plasma membrane and cytoplasm. "
- Primary Ab dilution
- 1:100