GTX25654

antibody from GeneTex

Targeting: ERBB2 CD340, HER-2, HER2, NEU, NGL

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX25654

Provider

GeneTex

Proper citation

GeneTex Cat#GTX25654, RRID:AB_380625

Product name

Her2 / ErbB2 (phospho Tyr1248) antibody

Antibody type

Polyclonal

Reactivity

Human, Rat, Canine, Hamster

Host

Rabbit

References

Submitted references

High cell-surface density of HER2 deforms cell membranes.

Chung I, Reichelt M, Shao L, Akita RW, Koeppen H, Rangell L, Schaefer G, Mellman I, Sliwkowski MX
Nature communications 2016 Sep 7;7:12742

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Extracts of SK-BR-3 cells were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was either not treated (1-5, 7) or treated (6, 8) with YOP phosphatase, blocked with a 5% BSA-TBST buffer overnight at 4¢XC, then incubated with the ErbB-2 [pY1248] antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 5-8), the non-phosphopeptide corresponding to the phosphopeptide immunogen (2), a generic phosphotyrosine-containing peptide (3), or the phosphopeptide immunogen (4). After washing, the membrane was incubated with goat F(ab)2 anti-rabbit IgG alkaline phosphatase and signals were detected.The data show that only the phosphopeptide corresponding to ErbB-2 [pY1248] blocks the antibody signal, demonstrating the specificity of the antibody. The data also show that phosphatase stripping eliminates the signal, further verifying that the antibody is phospho-specific.