AM00048PU-N

antibody from Acris Antibodies GmbH

Targeting: ERBB2 CD340, HER-2, HER2, NEU, NGL

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AM00048PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AM00048PU-N, RRID:AB_977164

Product name

anti CD340 / ERBB2 / HER2 pThr686 (incl. pos. control)

Antibody type

Monoclonal

Antigen

Phosphopeptide conjugated to KLH.Epitope: Phosphothreonine 686

Reactivity

Human, Mouse, Canine

Host

Mouse

Isotype

IgG

Antibody clone number

7F8

Vial size

0.1 mg

Concentration

0.1 mg/ml

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

ERBB2 Transactivation: Serum starved A431 cells were treated for 15 min as indicated . Whole cell lysates were separated by SDS-PAGE (ca 20.000 cells/lane). The immunoblot was probed with mab erbB2-7F8 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: ControlLane 2: PMALane 3: LPALane 4: CeramideLane 5: BradykininLane 6: Bombesin