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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- AM00052PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM00052PU-N, RRID:AB_977146
- Product name
- anti CD340 / ERBB2 / HER2 (1240-1260) (incl. pos. control)
- Antibody type
- Monoclonal
- Antigen
- Peptide conjugated to hemocyaninEpitope: aa 1240-1260
- Reactivity
- Human, Mouse, Canine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 19D2
- Vial size
- 0.1 mg
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Supportive validation
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- Acris Antibodies GmbH (provider)
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- Experimental details
- Detection of endogenous erbB2 Whole cell extracts of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab erbB2-19D2 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).lane 1: MDA-MB 231; lane 2: MDA-MB 468; lane 3: MCF-7; lane 4: T47D; lane 5: SW480; lane 6: SW620
