Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Flow cytometry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- ABIN2508065 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) antibody
- Antibody type
- Monoclonal
- Antigen
- This ERBB2 antibody is generated from a mouse immunized with a recombinant protein.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 200 μL
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of lysates from HL-60,A431,U-937 cell line (from left to right),using HRH4 Antibody(Cat. ABIN2511226). ABIN2511226 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane. Indirect flow cytometry analysis of H4 Histamine Receptor expression in live intact Jurkat (acute T-cell leukemia) cell lines Black: Unstained cells + goat-anti-rabbit-PE. Green: Cells+ Anti-Human H4 Histamine Receptor (extracellular) antibody (ABIN2511226), (1:20) + goat-anti-rabbit-PE. Flow cytometric analysis of Jurkat cells using Park7 (DJ-1) Antibody(green, CatABIN2511226) compared to an isotype control of rabbit IgG(blue). ABIN2511226 was diluted at 1:35 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of lysates from HL-60,A431,U-937 cell line (from left to right),using HRH4 Antibody(Cat. ABIN2511226). ABIN2511226 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane. Indirect flow cytometry analysis of H4 Histamine Receptor expression in live intact Jurkat (acute T-cell leukemia) cell lines Black: Unstained cells + goat-anti-rabbit-PE. Green: Cells+ Anti-Human H4 Histamine Receptor (extracellular) antibody (ABIN2511226), (1:20) + goat-anti-rabbit-PE. Flow cytometric analysis of Jurkat cells using Park7 (DJ-1) Antibody(green, CatABIN2511226) compared to an isotype control of rabbit IgG(blue). ABIN2511226 was diluted at 1:35 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Image(s): Flow cytometry
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Image(s): Flow cytometry