TA503503

antibody from OriGene

Targeting: ERBB2 CD340, HER-2, HER2, NEU, NGL

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA503503

Provider

OriGene

Proper citation

OriGene Cat#TA503503, RRID:AB_11127027

Product name

ERBB2 mouse monoclonal antibody, clone OTI1D9 (formerly 1D9)

Antibody type

Monoclonal

Description

ERBB2 mouse monoclonal antibody, clone OTI1D9 (formerly 1D9)

Host

Mouse

Conjugate

Unconjugated

Epitope

ERBB2

Isotype

IgG

Antibody clone number

OTI1D9

Vial size

100 µl

Concentration

0.23 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERBB2 (RC212583, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERBB2.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-ERBB2 mouse monoclonal antibody (TA503503) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERBB2(RC212583).

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-ERBB2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503503)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-ERBB2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503503)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ERBB2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503503)

Validation comment

IHC