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Western blot
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- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- ABIN359840 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (N-Term), (AA 30-59) antibody
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 30~59 amino acids from the N-terminal region of Human HER2/ErbB2 . Genename: ERBB2
- Description
- Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- N-Term,AA 30-59
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store the antibody undiluted at 2-8°C for one month or (in aliquots) at-20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Characterization of a new allele of the human ERBB2 gene by allele-specific competition hybridization.
Similarity of protein encoded by the human c-erb-B-2 gene to epidermal growth factor receptor.
A v-erbB-related protooncogene, c-erbB-2, is distinct from the c-erbB-1/epidermal growth factor-receptor gene and is amplified in a human salivary gland adenocarcinoma.
Tyrosine kinase receptor with extensive homology to EGF receptor shares chromosomal location with neu oncogene.
Ehsani A, Low J, Wallace RB, Wu AM
Genomics 1993 Feb;15(2):426-9
Genomics 1993 Feb;15(2):426-9
Similarity of protein encoded by the human c-erb-B-2 gene to epidermal growth factor receptor.
Yamamoto T, Ikawa S, Akiyama T, Semba K, Nomura N, Miyajima N, Saito T, Toyoshima K
Nature 1986 Jan 16-22;319(6050):230-4
Nature 1986 Jan 16-22;319(6050):230-4
A v-erbB-related protooncogene, c-erbB-2, is distinct from the c-erbB-1/epidermal growth factor-receptor gene and is amplified in a human salivary gland adenocarcinoma.
Semba K, Kamata N, Toyoshima K, Yamamoto T
Proceedings of the National Academy of Sciences of the United States of America 1985 Oct;82(19):6497-501
Proceedings of the National Academy of Sciences of the United States of America 1985 Oct;82(19):6497-501
Tyrosine kinase receptor with extensive homology to EGF receptor shares chromosomal location with neu oncogene.
Coussens L, Yang-Feng TL, Liao YC, Chen E, Gray A, McGrath J, Seeburg PH, Libermann TA, Schlessinger J, Francke U
Science (New York, N.Y.) 1985 Dec 6;230(4730):1132-9
Science (New York, N.Y.) 1985 Dec 6;230(4730):1132-9
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- (LEFT)Western blot analysis of anti-ErbB2 Pab in SKBR-3 cell lysate. ErbB2 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. (RIGHT)Western blot analysis of ErbB2 (arrow) using HER2/ErbB2 Antibody
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- (LEFT)Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. (RIGHT)Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with HER2/ErbB2 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Flow Cytometric analysis of MCF-7 cells using HER2/ErbB2 Antibody (N-term) Cat.-No AP14324PU-N (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
