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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [16]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- AF143 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Decorin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human Decorin in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse Decorin is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Absence of the proteoglycan decorin reduces glucose tolerance in overfed male mice.
Osteoblasts are "educated" by crosstalk with metastatic breast cancer cells in the bone tumor microenvironment.
Regulation of Myelination by Exosome Associated Retinoic Acid Release from NG2-Positive Cells.
Adenovirus-mediated decorin expression induces cancer cell death through activation of p53 and mitochondrial apoptosis.
Glucosamine exposure reduces proteoglycan synthesis in primary human endothelial cells in vitro.
Maternal obesity drives functional alterations in uterine NK cells.
MicroRNA 181b regulates decorin production by dermal fibroblasts and may be a potential therapy for hypertrophic scar.
Probing the acceptor active site organization of the human recombinant β1,4-galactosyltransferase 7 and design of xyloside-based inhibitors.
Resistance of corneal RFUVA–cross-linked collagens and small leucine-rich proteoglycans to degradation by matrix metalloproteinases.
Serglycin is a major proteoglycan in polarized human endothelial cells and is implicated in the secretion of the chemokine GROalpha/CXCL1.
Effects of ultraviolet-A and riboflavin on the interaction of collagen and proteoglycans during corneal cross-linking.
Adenovirus-mediated gene transfer of fibromodulin inhibits neointimal hyperplasia in an organ culture model of human saphenous vein graft disease.
Syndromic and non-syndromic aneurysms of the human ascending aorta share activation of the Smad2 pathway.
Dermatan sulfate proteoglycan biglycan as a potential selectin L/CD44 ligand involved in selective recruitment of peripheral blood CD16(-) natural killer cells into human endometrium.
Decorin is a secreted protein associated with obesity and type 2 diabetes.
Periodontitis destructions are restored by synthetic glycosaminoglycan mimetic.
Svärd J, Røst TH, Sommervoll CEN, Haugen C, Gudbrandsen OA, Mellgren AE, Rødahl E, Fernø J, Dankel SN, Sagen JV, Mellgren G
Scientific reports 2019 Mar 15;9(1):4614
Scientific reports 2019 Mar 15;9(1):4614
Osteoblasts are "educated" by crosstalk with metastatic breast cancer cells in the bone tumor microenvironment.
Kolb AD, Shupp AB, Mukhopadhyay D, Marini FC, Bussard KM
Breast cancer research : BCR 2019 Feb 27;21(1):31
Breast cancer research : BCR 2019 Feb 27;21(1):31
Regulation of Myelination by Exosome Associated Retinoic Acid Release from NG2-Positive Cells.
Goncalves MB, Wu Y, Clarke E, Grist J, Hobbs C, Trigo D, Jack J, Corcoran JPT
The Journal of neuroscience : the official journal of the Society for Neuroscience 2019 Apr 17;39(16):3013-3027
The Journal of neuroscience : the official journal of the Society for Neuroscience 2019 Apr 17;39(16):3013-3027
Adenovirus-mediated decorin expression induces cancer cell death through activation of p53 and mitochondrial apoptosis.
Yoon AR, Hong J, Yun CO
Oncotarget 2017 Sep 29;8(44):76666-76685
Oncotarget 2017 Sep 29;8(44):76666-76685
Glucosamine exposure reduces proteoglycan synthesis in primary human endothelial cells in vitro.
Reine TM, Jenssen TG, Kolset SO
Food & nutrition research 2016;60:32615
Food & nutrition research 2016;60:32615
Maternal obesity drives functional alterations in uterine NK cells.
Perdu S, Castellana B, Kim Y, Chan K, DeLuca L, Beristain AG
JCI insight 2016 Jul 21;1(11):e85560
JCI insight 2016 Jul 21;1(11):e85560
MicroRNA 181b regulates decorin production by dermal fibroblasts and may be a potential therapy for hypertrophic scar.
Kwan P, Ding J, Tredget EE
PloS one 2015;10(4):e0123054
PloS one 2015;10(4):e0123054
Probing the acceptor active site organization of the human recombinant β1,4-galactosyltransferase 7 and design of xyloside-based inhibitors.
Saliba M, Ramalanjaona N, Gulberti S, Bertin-Jung I, Thomas A, Dahbi S, Lopin-Bon C, Jacquinet JC, Breton C, Ouzzine M, Fournel-Gigleux S
The Journal of biological chemistry 2015 Mar 20;290(12):7658-70
The Journal of biological chemistry 2015 Mar 20;290(12):7658-70
Resistance of corneal RFUVA–cross-linked collagens and small leucine-rich proteoglycans to degradation by matrix metalloproteinases.
Zhang Y, Mao X, Schwend T, Littlechild S, Conrad GW
Investigative ophthalmology & visual science 2013 Feb 5;54(2):1014-25
Investigative ophthalmology & visual science 2013 Feb 5;54(2):1014-25
Serglycin is a major proteoglycan in polarized human endothelial cells and is implicated in the secretion of the chemokine GROalpha/CXCL1.
Meen AJ, Øynebråten I, Reine TM, Duelli A, Svennevig K, Pejler G, Jenssen T, Kolset SO
The Journal of biological chemistry 2011 Jan 28;286(4):2636-47
The Journal of biological chemistry 2011 Jan 28;286(4):2636-47
Effects of ultraviolet-A and riboflavin on the interaction of collagen and proteoglycans during corneal cross-linking.
Zhang Y, Conrad AH, Conrad GW
The Journal of biological chemistry 2011 Apr 15;286(15):13011-22
The Journal of biological chemistry 2011 Apr 15;286(15):13011-22
Adenovirus-mediated gene transfer of fibromodulin inhibits neointimal hyperplasia in an organ culture model of human saphenous vein graft disease.
Ranjzad P, Salem HK, Kingston PA
Gene therapy 2009 Sep;16(9):1154-62
Gene therapy 2009 Sep;16(9):1154-62
Syndromic and non-syndromic aneurysms of the human ascending aorta share activation of the Smad2 pathway.
Gomez D, Al Haj Zen A, Borges LF, Philippe M, Gutierrez PS, Jondeau G, Michel JB, Vranckx R
The Journal of pathology 2009 May;218(1):131-42
The Journal of pathology 2009 May;218(1):131-42
Dermatan sulfate proteoglycan biglycan as a potential selectin L/CD44 ligand involved in selective recruitment of peripheral blood CD16(-) natural killer cells into human endometrium.
Kitaya K, Yasuo T
Journal of leukocyte biology 2009 Mar;85(3):391-400
Journal of leukocyte biology 2009 Mar;85(3):391-400
Decorin is a secreted protein associated with obesity and type 2 diabetes.
Bolton K, Segal D, McMillan J, Jowett J, Heilbronn L, Abberton K, Zimmet P, Chisholm D, Collier G, Walder K
International journal of obesity (2005) 2008 Jul;32(7):1113-21
International journal of obesity (2005) 2008 Jul;32(7):1113-21
Periodontitis destructions are restored by synthetic glycosaminoglycan mimetic.
Lallam-Laroye C, Escartin Q, Zlowodzki AS, Barritault D, Caruelle JP, Baroukh B, Saffar JL, Colombier ML
Journal of biomedical materials research. Part A 2006 Dec 1;79(3):675-83
Journal of biomedical materials research. Part A 2006 Dec 1;79(3):675-83
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Decorin by Western Blot. Western blot shows lysates of human uterus tissue and human heart tissue. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human Decorin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF143) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Decorin at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Decorin by Simple WesternTM. Simple Western lane view shows lysates of human uterus and human heart tissue, loaded at 0.2 mg/mL. A specific band was detected for Decorin at approximately 140-149 kDa (as indicated) using 5 µg/mL of Goat Anti-Human Decorin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF143) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Decorin in Human Breast. Decorin was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human Decorin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF143) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to intralobular stromal tissue. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
