IC4013F

antibody from Novus Biologicals

Targeting: IL1B IL-1B, IL1-BETA, IL1F2

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

IC4013F

Provider

Novus Biologicals

Product name

Rat Monoclonal IL-1 beta/IL-1F2 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects mouse IL-1 beta/IL-1F2 in direct ELISAs. In direct ELISAs, approximately 75% cross-reactivity with recombinant guinea pig IL-1 beta , 5-15% cross-reactivitiy with recombinant canine, cotton rat, feline, equine, and rat IL-1 beta , and no cross-reactivity with recombinant human, porcine, and rhesus IL-1 beta is observed. No cross-reactivity with recombinant human (rh) Pro-IL-1 beta , rhIL-1F7, rhIL-1F10, rhIL-36 gamma , recombinant mouse (rm) IL-1 alpha , rmIL-18, rmIL-36Ra, rmIL-36 alpha , or rmIL-36 beta is observed.

Reactivity

Mouse

Host

Rat

Isotype

IgG

Vial size

100 Tests

Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of IL-1 beta/IL-1F2 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes, untreated (open histogram), or treated with LPS for 48 hours (filled histogram) were stained with Rat Anti-Mouse IL-1 beta/IL-1F2 Fluorescein-conjugated Monoclonal Antibody (Catalog # IC4013F, filled histogram) or isotype control antibody (Catalog # IC013F, data not shown). Histograms were gated on blasting splenocytes. To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.