Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- MAB4012 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Hamster Monoclonal IL-1 beta/IL-1F2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse IL-1 beta/IL-1 F2 in direct ELISAs and Western blots. In Western blots, shows 100% cross-reactivity with recombinant rat (rr) IL-1 beta and rcrIL-1 beta , 15%-35 % with rhIL-1 beta and rpIL-1 beta , and no cross-reactivity with rmIL-1 alpha , rmIL-18, or rhIL-1 beta rp.
- Reactivity
- Mouse
- Host
- Hamster
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Mycobacterium tuberculosis Limits Host Glycolysis and IL-1β by Restriction of PFK-M via MicroRNA-21.
Nicotinamide phosphoribosyltransferase aggravates inflammation and promotes atherosclerosis in ApoE knockout mice.
Cholinergic anti-inflammatory pathway inhibits neointimal hyperplasia by suppressing inflammation and oxidative stress.
Key Role of the Scavenger Receptor MARCO in Mediating Adenovirus Infection and Subsequent Innate Responses of Macrophages.
Cigarette smoke-induced accumulation of lung dendritic cells is interleukin-1α-dependent in mice.
IL-1α and IL-1β recruit different myeloid cells and promote different stages of sterile inflammation.
Hackett EE, Charles-Messance H, O'Leary SM, Gleeson LE, Muñoz-Wolf N, Case S, Wedderburn A, Johnston DGW, Williams MA, Smyth A, Ouimet M, Moore KJ, Lavelle EC, Corr SC, Gordon SV, Keane J, Sheedy FJ
Cell reports 2020 Jan 7;30(1):124-136.e4
Cell reports 2020 Jan 7;30(1):124-136.e4
Nicotinamide phosphoribosyltransferase aggravates inflammation and promotes atherosclerosis in ApoE knockout mice.
Kong YY, Li GQ, Zhang WJ, Hua X, Zhou CC, Xu TY, Li ZY, Wang P, Miao CY
Acta pharmacologica Sinica 2019 Sep;40(9):1184-1192
Acta pharmacologica Sinica 2019 Sep;40(9):1184-1192
Cholinergic anti-inflammatory pathway inhibits neointimal hyperplasia by suppressing inflammation and oxidative stress.
Li DJ, Fu H, Tong J, Li YH, Qu LF, Wang P, Shen FM
Redox biology 2018 May;15:22-33
Redox biology 2018 May;15:22-33
Key Role of the Scavenger Receptor MARCO in Mediating Adenovirus Infection and Subsequent Innate Responses of Macrophages.
Maler MD, Nielsen PJ, Stichling N, Cohen I, Ruzsics Z, Wood C, Engelhard P, Suomalainen M, Gyory I, Huber M, Müller-Quernheim J, Schamel WWA, Gordon S, Jakob T, Martin SF, Jahnen-Dechent W, Greber UF, Freudenberg MA, Fejer G
mBio 2017 Aug 1;8(4)
mBio 2017 Aug 1;8(4)
Cigarette smoke-induced accumulation of lung dendritic cells is interleukin-1α-dependent in mice.
Botelho FM, Nikota JK, Bauer CM, Morissette MC, Iwakura Y, Kolbeck R, Finch D, Humbles AA, Stämpfli MR
Respiratory research 2012 Sep 19;13:81
Respiratory research 2012 Sep 19;13:81
IL-1α and IL-1β recruit different myeloid cells and promote different stages of sterile inflammation.
Rider P, Carmi Y, Guttman O, Braiman A, Cohen I, Voronov E, White MR, Dinarello CA, Apte RN
Journal of immunology (Baltimore, Md. : 1950) 2011 Nov 1;187(9):4835-43
Journal of immunology (Baltimore, Md. : 1950) 2011 Nov 1;187(9):4835-43
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-1 beta/IL-1F2 and Neutralization by Mouse IL-1 beta/IL-1F2 Antibody. Recombinant Mouse IL-1 beta/IL-1F2 (Catalog # 401-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-1 beta/IL-1F2 (50 pg/mL) is neutralized (green line) by increasing concentrations of Mouse IL-1 beta/IL-1F2 Monoclonal Antibody (Catalog # MAB4012). The ND50 is typically 0.3-1.5 µg/mL in the presence of concanavalin A (1.25 µg/mL).