Antibody data
- Antibody Data
- Antigen structure
- References [23]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- MAB601-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-1 beta/IL-1F2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human IL-1 beta/IL-1F2 in sandwich ELISAs and Western blots. In sandwich ELISAs, less than 4% cross-reactivity with recombinant rat (rr) IL-1 beta and less than 0.1% with recombinant porcine (rp) IL-1 beta , recombinant human IL-1 alpha , rpIL-1 alpha , rrIL-1 alpha , recombinant mouse (rm) IL-1 alpha , and rmIL-1 beta is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 2805
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Heterogeneous GM-CSF signaling in macrophages is associated with control of Mycobacterium tuberculosis.
Targeting the miR-665-3p-ATG4B-autophagy axis relieves inflammation and apoptosis in intestinal ischemia/reperfusion.
Exposure to wild-type AAV drives distinct capsid immunity profiles in humans.
DPP8/DPP9 inhibitor-induced pyroptosis for treatment of acute myeloid leukemia.
Saturated Fatty Acids Undergo Intracellular Crystallization and Activate the NLRP3 Inflammasome in Macrophages.
Innate activation of human primary epithelial cells broadens the host response to Mycobacterium tuberculosis in the airways.
Zinc inhibits high glucose-induced NLRP3 inflammasome activation in human peritoneal mesothelial cells.
Vitamin D regulates cytokine patterns secreted by dendritic cells to promote differentiation of IL-22-producing T cells.
Alpha 1-antitrypsin does not inhibit human monocyte caspase-1.
Inflammasome priming by lipopolysaccharide is dependent upon ERK signaling and proteasome function.
Measurement of interleukin-33 (IL-33) and IL-33 receptors (sST2 and ST2L) in patients with rheumatoid arthritis.
IL-17C regulates the innate immune function of epithelial cells in an autocrine manner.
Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
Bacterial vaginosis, not HIV, is primarily responsible for increased vaginal concentrations of proinflammatory cytokines.
Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
Endogenous IL-32 controls cytokine and HIV-1 production.
Ultrasensitive flow-based immunoassays using single-molecule counting.
Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1.
Clinical outcome following acute ischaemic stroke relates to both activation and autoregulatory inhibition of cytokine production.
Changes in periodontal and rheumatological conditions after 2 years in patients with juvenile idiopathic arthritis.
Rapid analysis of inflammatory cytokines in cerebrospinal fluid using chip-based immunoaffinity electrophoresis.
Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines.
Bryson BD, Rosebrock TR, Tafesse FG, Itoh CY, Nibasumba A, Babunovic GH, Corleis B, Martin C, Keegan C, Andrade P, Realegeno S, Kwon D, Modlin RL, Fortune SM
Nature communications 2019 May 27;10(1):2329
Nature communications 2019 May 27;10(1):2329
Targeting the miR-665-3p-ATG4B-autophagy axis relieves inflammation and apoptosis in intestinal ischemia/reperfusion.
Li Z, Wang G, Feng D, Zu G, Li Y, Shi X, Zhao Y, Jing H, Ning S, Le W, Yao J, Tian X
Cell death & disease 2018 May 1;9(5):483
Cell death & disease 2018 May 1;9(5):483
Exposure to wild-type AAV drives distinct capsid immunity profiles in humans.
Kuranda K, Jean-Alphonse P, Leborgne C, Hardet R, Collaud F, Marmier S, Costa Verdera H, Ronzitti G, Veron P, Mingozzi F
The Journal of clinical investigation 2018 Dec 3;128(12):5267-5279
The Journal of clinical investigation 2018 Dec 3;128(12):5267-5279
DPP8/DPP9 inhibitor-induced pyroptosis for treatment of acute myeloid leukemia.
Johnson DC, Taabazuing CY, Okondo MC, Chui AJ, Rao SD, Brown FC, Reed C, Peguero E, de Stanchina E, Kentsis A, Bachovchin DA
Nature medicine 2018 Aug;24(8):1151-1156
Nature medicine 2018 Aug;24(8):1151-1156
Saturated Fatty Acids Undergo Intracellular Crystallization and Activate the NLRP3 Inflammasome in Macrophages.
Karasawa T, Kawashima A, Usui-Kawanishi F, Watanabe S, Kimura H, Kamata R, Shirasuna K, Koyama Y, Sato-Tomita A, Matsuzaka T, Tomoda H, Park SY, Shibayama N, Shimano H, Kasahara T, Takahashi M
Arteriosclerosis, thrombosis, and vascular biology 2018 Apr;38(4):744-756
Arteriosclerosis, thrombosis, and vascular biology 2018 Apr;38(4):744-756
Innate activation of human primary epithelial cells broadens the host response to Mycobacterium tuberculosis in the airways.
Reuschl AK, Edwards MR, Parker R, Connell DW, Hoang L, Halliday A, Jarvis H, Siddiqui N, Wright C, Bremang S, Newton SM, Beverley P, Shattock RJ, Kon OM, Lalvani A
PLoS pathogens 2017 Sep;13(9):e1006577
PLoS pathogens 2017 Sep;13(9):e1006577
Zinc inhibits high glucose-induced NLRP3 inflammasome activation in human peritoneal mesothelial cells.
Fan Y, Zhang X, Yang L, Wang J, Hu Y, Bian A, Liu J, Ma J
Molecular medicine reports 2017 Oct;16(4):5195-5202
Molecular medicine reports 2017 Oct;16(4):5195-5202
Vitamin D regulates cytokine patterns secreted by dendritic cells to promote differentiation of IL-22-producing T cells.
Sommer A, Fabri M
PloS one 2015;10(6):e0130395
PloS one 2015;10(6):e0130395
Alpha 1-antitrypsin does not inhibit human monocyte caspase-1.
Rahman MA, Mitra S, Sarkar A, Wewers MD
PloS one 2015;10(2):e0117330
PloS one 2015;10(2):e0117330
Inflammasome priming by lipopolysaccharide is dependent upon ERK signaling and proteasome function.
Ghonime MG, Shamaa OR, Das S, Eldomany RA, Fernandes-Alnemri T, Alnemri ES, Gavrilin MA, Wewers MD
Journal of immunology (Baltimore, Md. : 1950) 2014 Apr 15;192(8):3881-8
Journal of immunology (Baltimore, Md. : 1950) 2014 Apr 15;192(8):3881-8
Measurement of interleukin-33 (IL-33) and IL-33 receptors (sST2 and ST2L) in patients with rheumatoid arthritis.
Hong YS, Moon SJ, Joo YB, Jeon CH, Cho ML, Ju JH, Oh HJ, Heo YJ, Park SH, Kim HY, Min JK
Journal of Korean medical science 2011 Sep;26(9):1132-9
Journal of Korean medical science 2011 Sep;26(9):1132-9
IL-17C regulates the innate immune function of epithelial cells in an autocrine manner.
Ramirez-Carrozzi V, Sambandam A, Luis E, Lin Z, Jeet S, Lesch J, Hackney J, Kim J, Zhou M, Lai J, Modrusan Z, Sai T, Lee W, Xu M, Caplazi P, Diehl L, de Voss J, Balazs M, Gonzalez L Jr, Singh H, Ouyang W, Pappu R
Nature immunology 2011 Oct 12;12(12):1159-66
Nature immunology 2011 Oct 12;12(12):1159-66
Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
Hu H, Kwun J, Aizenstein BD, Knechtle SJ
Transplantation 2009 Jun 27;87(12):1814-20
Transplantation 2009 Jun 27;87(12):1814-20
Bacterial vaginosis, not HIV, is primarily responsible for increased vaginal concentrations of proinflammatory cytokines.
Mitchell CM, Balkus J, Agnew KJ, Cohn S, Luque A, Lawler R, Coombs RW, Hitti JE
AIDS research and human retroviruses 2008 May;24(5):667-71
AIDS research and human retroviruses 2008 May;24(5):667-71
Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB
AIDS research and human retroviruses 2008 Mar;24(3):453-62
AIDS research and human retroviruses 2008 Mar;24(3):453-62
Endogenous IL-32 controls cytokine and HIV-1 production.
Nold MF, Nold-Petry CA, Pott GB, Zepp JA, Saavedra MT, Kim SH, Dinarello CA
Journal of immunology (Baltimore, Md. : 1950) 2008 Jul 1;181(1):557-65
Journal of immunology (Baltimore, Md. : 1950) 2008 Jul 1;181(1):557-65
Ultrasensitive flow-based immunoassays using single-molecule counting.
Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, Goix P
Clinical chemistry 2007 Nov;53(11):1990-5
Clinical chemistry 2007 Nov;53(11):1990-5
Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
Blood 2007 May 15;109(10):4272-9
Blood 2007 May 15;109(10):4272-9
HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1.
Grivel JC, Elliott J, Lisco A, Biancotto A, Condack C, Shattock RJ, McGowan I, Margolis L, Anton P
AIDS (London, England) 2007 Jun 19;21(10):1263-72
AIDS (London, England) 2007 Jun 19;21(10):1263-72
Clinical outcome following acute ischaemic stroke relates to both activation and autoregulatory inhibition of cytokine production.
Emsley HC, Smith CJ, Gavin CM, Georgiou RF, Vail A, Barberan EM, Illingworth K, Scarth S, Wickramasinghe V, Hoadley ME, Rothwell NJ, Tyrrell PJ, Hopkins SJ
BMC neurology 2007 Feb 28;7:5
BMC neurology 2007 Feb 28;7:5
Changes in periodontal and rheumatological conditions after 2 years in patients with juvenile idiopathic arthritis.
Miranda LA, Braga F, Fischer RG, Sztajnbok FR, Figueredo CM, Gustafsson A
Journal of periodontology 2006 Oct;77(10):1695-700
Journal of periodontology 2006 Oct;77(10):1695-700
Rapid analysis of inflammatory cytokines in cerebrospinal fluid using chip-based immunoaffinity electrophoresis.
Phillips TM
Electrophoresis 2004 Jun;25(10-11):1652-9
Electrophoresis 2004 Jun;25(10-11):1652-9
Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines.
Copeland S, Siddiqui J, Remick D
Journal of immunological methods 2004 Jan;284(1-2):99-106
Journal of immunological methods 2004 Jan;284(1-2):99-106
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human IL-1 beta/IL-1F2 by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM PMA for 24 hours and 10 µg/mL LPS and 3 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human IL-1 beta/IL-1F2 Monoclonal Antibody (Catalog # MAB601) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IL-1 beta/IL-1F2 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-1 beta/IL-1F2 in Human PBMCs. IL-1 beta/IL-1F2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human IL-1 beta/IL-1F2 Monoclonal Antibody (Catalog # MAB601) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-1 beta/IL-1F2 and Neutralization by Human IL-1 beta/IL-1F2 Antibody. Recombinant Human IL-1 beta/IL-1F2 (Catalog # 201-LB) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-1 beta/IL-1F2 (0.05 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-1 beta/IL-1F2 Monoclonal Antibody (Catalog # MAB601). The ND50 is typically 0.05-0.2 µg/mL in the presence of concanavalin A (1.25 µg/mL).