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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF681 - Provider product page
- Provider
- R&D Systems
- Product name
- Porcine IL-1 beta/IL-1F2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects porcine IL-1 beta/IL-1F2 in direct ELISAs and Western blots. In direct ELISAs, approximately 45%-75% cross-reactivity with recombinant human IL-1 beta , recombinant mouse IL-1 beta , recombinant rat IL-1 beta , recombinant feline IL-1 beta , recombinant canine IL-1 beta , recombinant equine IL-1 beta , recombinant guinea pig IL-1 beta , recombinant rabbit IL-1 beta , and recombinant rhesus monkey IL-1 beta is observed and less than 15% cross-reactivity with recombinant cotton rat IL-1 beta is observed.
- Reactivity
- Porcine
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P26889- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references NS1 Protein of 2009 Pandemic Influenza A Virus Inhibits Porcine NLRP3 Inflammasome-Mediated Interleukin-1 Beta Production by Suppressing ASC Ubiquitination.
Atorvastatin treatment improves survival and effects of implanted mesenchymal stem cells in post-infarct swine hearts.
Induction of interleukin-1 in articular cartilage by explantation and cutting.
Park HS, Liu G, Thulasi Raman SN, Landreth SL, Liu Q, Zhou Y
Journal of virology 2018 Apr 15;92(8)
Journal of virology 2018 Apr 15;92(8)
Atorvastatin treatment improves survival and effects of implanted mesenchymal stem cells in post-infarct swine hearts.
Yang YJ, Qian HY, Huang J, Geng YJ, Gao RL, Dou KF, Yang GS, Li JJ, Shen R, He ZX, Lu MJ, Zhao SH
European heart journal 2008 Jun;29(12):1578-90
European heart journal 2008 Jun;29(12):1578-90
Induction of interleukin-1 in articular cartilage by explantation and cutting.
Gruber J, Vincent TL, Hermansson M, Bolton M, Wait R, Saklatvala J
Arthritis and rheumatism 2004 Aug;50(8):2539-46
Arthritis and rheumatism 2004 Aug;50(8):2539-46
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Cell Proliferation Induced by IL-1 beta/IL-1F2 and Neutrali-zation by Porcine IL-1 beta/IL-1F2 Antibody. Recombinant Porcine IL-1 beta/IL-1F2 (Catalog # 681-PI) stimulates proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL-1 beta/IL-1F2 (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine IL-1 beta/ IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF681). The ND50 is typically 4-20 µg/mL in the presence of concanavalin A (1.25 µg/mL).
