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- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- PAB11294 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB11294, RRID:AB_1676672
- Product name
- IL1B polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein corresponding to 153 amino acids of human IL1B.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 1 mg
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Sandwich ELISA formats designed to detect 17 kDa IL-1 beta significantly underestimate 35 kDa IL-1 beta.
A novel heterodimeric cysteine protease is required for interleukin-1 beta processing in monocytes.
Molecular cloning of the interleukin-1 beta converting enzyme.
Herzyk DJ, Berger AE, Allen JN, Wewers MD
Journal of immunological methods 1992 Apr 8;148(1-2):243-54
Journal of immunological methods 1992 Apr 8;148(1-2):243-54
A novel heterodimeric cysteine protease is required for interleukin-1 beta processing in monocytes.
Thornberry NA, Bull HG, Calaycay JR, Chapman KT, Howard AD, Kostura MJ, Miller DK, Molineaux SM, Weidner JR, Aunins J
Nature 1992 Apr 30;356(6372):768-74
Nature 1992 Apr 30;356(6372):768-74
Molecular cloning of the interleukin-1 beta converting enzyme.
Cerretti DP, Kozlosky CJ, Mosley B, Nelson N, Van Ness K, Greenstreet TA, March CJ, Kronheim SR, Druck T, Cannizzaro LA
Science (New York, N.Y.) 1992 Apr 3;256(5053):97-100
Science (New York, N.Y.) 1992 Apr 3;256(5053):97-100
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoblotting of IL1B polyclonal antibody (Cat # PAB11294) was used at a 1 : 200 dilution incubated 1 h at room temperature to detect dog IL1B by Western blot.Lane 1 and 2 were loaded with 2.5 ug and 500 ng of dog IL1B respectively.The molecular weight of the detected band is estimated by comparison to molecular weight markers (not shown).Detection occurred using a 1 : 3,000 dilution of IRDye™800 conjugated Donkey anti-Rabbit IgG for 1h at room temperature.LICOR's Odyssey® Infrared Imaging System was used to scan and process the image.
- Validation comment
- Western Blot (Recombinant protein)
