Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PAB16926 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB16926, RRID:AB_10676577
- Product name
- IL1B polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against full length recombinant IL1B.
- Storage
- Store at 4°C on dry atmosphere.After reconstitution with 0.1 mL of deionized water, store at -20°C or lower.Aliquot to avoid repeated freezing and thawing.
Submitted references Molecular cloning of the interleukin-1 beta converting enzyme.
Nucleotide sequence of human monocyte interleukin 1 precursor cDNA.
Cerretti DP, Kozlosky CJ, Mosley B, Nelson N, Van Ness K, Greenstreet TA, March CJ, Kronheim SR, Druck T, Cannizzaro LA
Science (New York, N.Y.) 1992 Apr 3;256(5053):97-100
Science (New York, N.Y.) 1992 Apr 3;256(5053):97-100
Nucleotide sequence of human monocyte interleukin 1 precursor cDNA.
Auron PE, Webb AC, Rosenwasser LJ, Mucci SF, Rich A, Wolff SM, Dinarello CA
Proceedings of the National Academy of Sciences of the United States of America 1984 Dec;81(24):7907-11
Proceedings of the National Academy of Sciences of the United States of America 1984 Dec;81(24):7907-11
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot showing detection of recombinant human IL1B by IL1B polyclonal antibody (Cat # PAB16926). Recombinant human IL1B was loaded on to an SDS-PAGE gel at 0.1 ug and after separation, transferred to nitrocellulose. The expected band is approximately 18 KDa in size. The membrane was blocked with 1% BSA in TBST for 30min at RT, followed by incubation with primary antibody diluted 1 : 1,000 in1% BSA in TBST overnight at 4°C. After washes, the blot was reacted with secondary antibody HRP Goat anti-Rabbit IgG antibody diluted 1 : 40,000 in blocking buffer for 30 min at RT. Data was collected using Bio-Rad Versa Doc® 4000 MP imaging system.
