Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- A00101-2 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-IL-1 beta Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal antibody for IL1B detection. Tested positive for ELISA,Immunohistochemistry,Immunoprecipitation,Western Blot, in human, primate, dog
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 1mg
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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Supportive validation
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- Boster Biological Technology (provider)
- Main image
- Experimental details
- IHC analysis of IL1B using anti-IL1B antibody (A00101-2).IL1B was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-IL1B Antibody (A00101-2) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
- Additional image