A00101-3

antibody from Boster Biological Technology

Targeting: IL1B IL-1B, IL1-BETA, IL1F2

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

A00101-3

Provider

Boster Biological Technology

Product name

Anti-IL1 beta Antibody

Antibody type

Polyclonal

Description

Rabbit Polyclonal antibody for Il1b detection. Tested positive for ELISA,Flow Cytometry,Immunohistochemistry,IF Microscopy,Immunoprecipitation,Western Blot, in mouse and rat

Reactivity

Mouse, Rat

Host

Rabbit

Vial size

100ug

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

Store Anti-IL-1 beta antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

IHC analysis of Il1b using anti-Il1b antibody (A00101-3).Il1b was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Il1b Antibody (A00101-3) overnight at 4 °C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37 °C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image