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- Product number
- GTX74034 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX74034, RRID:AB_378141
- Product name
- IL1 beta antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Vildagliptin Reduces Stenosis of Injured Carotid Artery in Diabetic Mouse Through Inhibiting Vascular Smooth Muscle Cell Proliferation via ER Stress/NF-κB Pathway.
In vitro studies on anti-inflammatory activities of kiwifruit peel extract in human THP-1 monocytes.
CPTP: A sphingolipid transfer protein that regulates autophagy and inflammasome activation.
Structural basis for specific flagellin recognition by the NLR protein NAIP5.
The Pore-Forming Protein Gasdermin D Regulates Interleukin-1 Secretion from Living Macrophages.
ASC- and caspase-8-dependent apoptotic pathway diverges from the NLRC4 inflammasome in macrophages.
Increased Prevalence of Type 2 Diabetes-Related Complications in Combined Type 2 Diabetes and Sickle Cell Trait.
NLRP3 lacking the leucine-rich repeat domain can be fully activated via the canonical inflammasome pathway.
Iron overloaded polarizes macrophage to proinflammation phenotype through ROS/acetyl-p53 pathway.
Novel Indole-fused benzo-oxazepines (IFBOs) inhibit invasion of hepatocellular carcinoma by targeting IL-6 mediated JAK2/STAT3 oncogenic signals.
Anti-inflammation performance of curcumin-loaded mesoporous calcium silicate cement.
MyD88-dependent inflammasome activation and autophagy inhibition contributes to Ehrlichia-induced liver injury and toxic shock.
By Capturing Inflammatory Lipids Released from Dying Cells, the Receptor CD14 Induces Inflammasome-Dependent Phagocyte Hyperactivation.
Neutrophilic NLRP3 inflammasome-dependent IL-1β secretion regulates the γδT17 cell response in respiratory bacterial infections.
VHL Inactivation in Precancerous Kidney Cells Induces an Inflammatory Response via ER Stress-Activated IRE1α Signaling.
Clonal hematopoiesis associated with TET2 deficiency accelerates atherosclerosis development in mice.
Caspase-1 Engagement and TLR-Induced c-FLIP Expression Suppress ASC/Caspase-8-Dependent Apoptosis by Inflammasome Sensors NLRP1b and NLRC4.
NLRP3 recruitment by NLRC4 during Salmonella infection.
An endogenous caspase-11 ligand elicits interleukin-1 release from living dendritic cells.
Familial Mediterranean fever mutations lift the obligatory requirement for microtubules in Pyrin inflammasome activation.
Differential ASC requirements reveal a key role for neutrophils and a noncanonical IL-1β response to Pseudomonas aeruginosa.
The ketone metabolite β-hydroxybutyrate blocks NLRP3 inflammasome-mediated inflammatory disease.
Caspase-8 scaffolding function and MLKL regulate NLRP3 inflammasome activation downstream of TLR3.
Macrophage-specific de Novo Synthesis of Ceramide Is Dispensable for Inflammasome-driven Inflammation and Insulin Resistance in Obesity.
Activation of the NLRP1b inflammasome independently of ASC-mediated caspase-1 autoproteolysis and speck formation.
Acidosis potentiates the host proinflammatory interleukin-1β response to Pseudomonas aeruginosa infection.
Negative regulation of the NLRP3 inflammasome by A20 protects against arthritis.
SIGIRR, a negative regulator of TLR/IL-1R signalling promotes Microbiota dependent resistance to colonization by enteric bacterial pathogens.
Non-canonical inflammasome activation targets caspase-11.
Ji Y, Ge Y, Xu X, Ye S, Fan Y, Zhang J, Mei L, Zhang X, Ying L, Yang T, Li C
Frontiers in pharmacology 2019;10:142
Frontiers in pharmacology 2019;10:142
In vitro studies on anti-inflammatory activities of kiwifruit peel extract in human THP-1 monocytes.
D'Eliseo D, Pannucci E, Bernini R, Campo M, Romani A, Santi L, Velotti F
Journal of ethnopharmacology 2019 Apr 6;233:41-46
Journal of ethnopharmacology 2019 Apr 6;233:41-46
CPTP: A sphingolipid transfer protein that regulates autophagy and inflammasome activation.
Mishra SK, Gao YG, Deng Y, Chalfant CE, Hinchcliffe EH, Brown RE
Autophagy 2018;14(5):862-879
Autophagy 2018;14(5):862-879
Structural basis for specific flagellin recognition by the NLR protein NAIP5.
Yang X, Yang F, Wang W, Lin G, Hu Z, Han Z, Qi Y, Zhang L, Wang J, Sui SF, Chai J
Cell research 2018 Jan;28(1):35-47
Cell research 2018 Jan;28(1):35-47
The Pore-Forming Protein Gasdermin D Regulates Interleukin-1 Secretion from Living Macrophages.
Evavold CL, Ruan J, Tan Y, Xia S, Wu H, Kagan JC
Immunity 2018 Jan 16;48(1):35-44.e6
Immunity 2018 Jan 16;48(1):35-44.e6
ASC- and caspase-8-dependent apoptotic pathway diverges from the NLRC4 inflammasome in macrophages.
Lee BL, Mirrashidi KM, Stowe IB, Kummerfeld SK, Watanabe C, Haley B, Cuellar TL, Reichelt M, Kayagaki N
Scientific reports 2018 Feb 28;8(1):3788
Scientific reports 2018 Feb 28;8(1):3788
Increased Prevalence of Type 2 Diabetes-Related Complications in Combined Type 2 Diabetes and Sickle Cell Trait.
Skinner SC, Diaw M, Pialoux V, Mbaye MN, Mury P, Lopez P, Bousquet D, Gueye F, Diedhiou D, Joly P, Renoux C, Sow D, Diop S, Ranque B, Vinet A, Samb A, Guillot N, Connes P
Diabetes care 2018 Dec;41(12):2595-2602
Diabetes care 2018 Dec;41(12):2595-2602
NLRP3 lacking the leucine-rich repeat domain can be fully activated via the canonical inflammasome pathway.
Hafner-Bratkovič I, Sušjan P, Lainšček D, Tapia-Abellán A, Cerović K, Kadunc L, Angosto-Bazarra D, Pelegrin P, Jerala R
Nature communications 2018 Dec 5;9(1):5182
Nature communications 2018 Dec 5;9(1):5182
Iron overloaded polarizes macrophage to proinflammation phenotype through ROS/acetyl-p53 pathway.
Zhou Y, Que KT, Zhang Z, Yi ZJ, Zhao PX, You Y, Gong JP, Liu ZJ
Cancer medicine 2018 Aug;7(8):4012-4022
Cancer medicine 2018 Aug;7(8):4012-4022
Novel Indole-fused benzo-oxazepines (IFBOs) inhibit invasion of hepatocellular carcinoma by targeting IL-6 mediated JAK2/STAT3 oncogenic signals.
Singh AK, Bhadauria AS, Kumar U, Raj V, Rai A, Kumar P, Keshari AK, Kumar D, Maity B, Nath S, Prakash A, Saha S
Scientific reports 2018 Apr 12;8(1):5932
Scientific reports 2018 Apr 12;8(1):5932
Anti-inflammation performance of curcumin-loaded mesoporous calcium silicate cement.
Chen YC, Shie MY, Wu YA, Lee KA, Wei LJ, Shen YF
Journal of the Formosan Medical Association = Taiwan yi zhi 2017 Sep;116(9):679-688
Journal of the Formosan Medical Association = Taiwan yi zhi 2017 Sep;116(9):679-688
MyD88-dependent inflammasome activation and autophagy inhibition contributes to Ehrlichia-induced liver injury and toxic shock.
Kader M, Alaoui-El-Azher M, Vorhauer J, Kode BB, Wells JZ, Stolz D, Michalopoulos G, Wells A, Scott M, Ismail N
PLoS pathogens 2017 Oct;13(10):e1006644
PLoS pathogens 2017 Oct;13(10):e1006644
By Capturing Inflammatory Lipids Released from Dying Cells, the Receptor CD14 Induces Inflammasome-Dependent Phagocyte Hyperactivation.
Zanoni I, Tan Y, Di Gioia M, Springstead JR, Kagan JC
Immunity 2017 Oct 17;47(4):697-709.e3
Immunity 2017 Oct 17;47(4):697-709.e3
Neutrophilic NLRP3 inflammasome-dependent IL-1β secretion regulates the γδT17 cell response in respiratory bacterial infections.
Hassane M, Demon D, Soulard D, Fontaine J, Keller LE, Patin EC, Porte R, Prinz I, Ryffel B, Kadioglu A, Veening JW, Sirard JC, Faveeuw C, Lamkanfi M, Trottein F, Paget C
Mucosal immunology 2017 Jul;10(4):1056-1068
Mucosal immunology 2017 Jul;10(4):1056-1068
VHL Inactivation in Precancerous Kidney Cells Induces an Inflammatory Response via ER Stress-Activated IRE1α Signaling.
Kuo CY, Lin CH, Hsu T
Cancer research 2017 Jul 1;77(13):3406-3416
Cancer research 2017 Jul 1;77(13):3406-3416
Clonal hematopoiesis associated with TET2 deficiency accelerates atherosclerosis development in mice.
Fuster JJ, MacLauchlan S, Zuriaga MA, Polackal MN, Ostriker AC, Chakraborty R, Wu CL, Sano S, Muralidharan S, Rius C, Vuong J, Jacob S, Muralidhar V, Robertson AA, Cooper MA, Andrés V, Hirschi KK, Martin KA, Walsh K
Science (New York, N.Y.) 2017 Feb 24;355(6327):842-847
Science (New York, N.Y.) 2017 Feb 24;355(6327):842-847
Caspase-1 Engagement and TLR-Induced c-FLIP Expression Suppress ASC/Caspase-8-Dependent Apoptosis by Inflammasome Sensors NLRP1b and NLRC4.
Van Opdenbosch N, Van Gorp H, Verdonckt M, Saavedra PHV, de Vasconcelos NM, Gonçalves A, Vande Walle L, Demon D, Matusiak M, Van Hauwermeiren F, D'Hont J, Hochepied T, Krautwald S, Kanneganti TD, Lamkanfi M
Cell reports 2017 Dec 19;21(12):3427-3444
Cell reports 2017 Dec 19;21(12):3427-3444
NLRP3 recruitment by NLRC4 during Salmonella infection.
Qu Y, Misaghi S, Newton K, Maltzman A, Izrael-Tomasevic A, Arnott D, Dixit VM
The Journal of experimental medicine 2016 May 30;213(6):877-85
The Journal of experimental medicine 2016 May 30;213(6):877-85
An endogenous caspase-11 ligand elicits interleukin-1 release from living dendritic cells.
Zanoni I, Tan Y, Di Gioia M, Broggi A, Ruan J, Shi J, Donado CA, Shao F, Wu H, Springstead JR, Kagan JC
Science (New York, N.Y.) 2016 Jun 3;352(6290):1232-6
Science (New York, N.Y.) 2016 Jun 3;352(6290):1232-6
Familial Mediterranean fever mutations lift the obligatory requirement for microtubules in Pyrin inflammasome activation.
Van Gorp H, Saavedra PH, de Vasconcelos NM, Van Opdenbosch N, Vande Walle L, Matusiak M, Prencipe G, Insalaco A, Van Hauwermeiren F, Demon D, Bogaert DJ, Dullaers M, De Baere E, Hochepied T, Dehoorne J, Vermaelen KY, Haerynck F, De Benedetti F, Lamkanfi M
Proceedings of the National Academy of Sciences of the United States of America 2016 Dec 13;113(50):14384-14389
Proceedings of the National Academy of Sciences of the United States of America 2016 Dec 13;113(50):14384-14389
Differential ASC requirements reveal a key role for neutrophils and a noncanonical IL-1β response to Pseudomonas aeruginosa.
Patankar YR, Mabaera R, Berwin B
American journal of physiology. Lung cellular and molecular physiology 2015 Oct 15;309(8):L902-13
American journal of physiology. Lung cellular and molecular physiology 2015 Oct 15;309(8):L902-13
The ketone metabolite β-hydroxybutyrate blocks NLRP3 inflammasome-mediated inflammatory disease.
Youm YH, Nguyen KY, Grant RW, Goldberg EL, Bodogai M, Kim D, D'Agostino D, Planavsky N, Lupfer C, Kanneganti TD, Kang S, Horvath TL, Fahmy TM, Crawford PA, Biragyn A, Alnemri E, Dixit VD
Nature medicine 2015 Mar;21(3):263-9
Nature medicine 2015 Mar;21(3):263-9
Caspase-8 scaffolding function and MLKL regulate NLRP3 inflammasome activation downstream of TLR3.
Kang S, Fernandes-Alnemri T, Rogers C, Mayes L, Wang Y, Dillon C, Roback L, Kaiser W, Oberst A, Sagara J, Fitzgerald KA, Green DR, Zhang J, Mocarski ES, Alnemri ES
Nature communications 2015 Jun 24;6:7515
Nature communications 2015 Jun 24;6:7515
Macrophage-specific de Novo Synthesis of Ceramide Is Dispensable for Inflammasome-driven Inflammation and Insulin Resistance in Obesity.
Camell CD, Nguyen KY, Jurczak MJ, Christian BE, Shulman GI, Shadel GS, Dixit VD
The Journal of biological chemistry 2015 Dec 4;290(49):29402-13
The Journal of biological chemistry 2015 Dec 4;290(49):29402-13
Activation of the NLRP1b inflammasome independently of ASC-mediated caspase-1 autoproteolysis and speck formation.
Van Opdenbosch N, Gurung P, Vande Walle L, Fossoul A, Kanneganti TD, Lamkanfi M
Nature communications 2014;5:3209
Nature communications 2014;5:3209
Acidosis potentiates the host proinflammatory interleukin-1β response to Pseudomonas aeruginosa infection.
Torres IM, Patankar YR, Shabaneh TB, Dolben E, Hogan DA, Leib DA, Berwin BL
Infection and immunity 2014 Nov;82(11):4689-97
Infection and immunity 2014 Nov;82(11):4689-97
Negative regulation of the NLRP3 inflammasome by A20 protects against arthritis.
Vande Walle L, Van Opdenbosch N, Jacques P, Fossoul A, Verheugen E, Vogel P, Beyaert R, Elewaut D, Kanneganti TD, van Loo G, Lamkanfi M
Nature 2014 Aug 7;512(7512):69-73
Nature 2014 Aug 7;512(7512):69-73
SIGIRR, a negative regulator of TLR/IL-1R signalling promotes Microbiota dependent resistance to colonization by enteric bacterial pathogens.
Sham HP, Yu EY, Gulen MF, Bhinder G, Stahl M, Chan JM, Brewster L, Morampudi V, Gibson DL, Hughes MR, McNagny KM, Li X, Vallance BA
PLoS pathogens 2013;9(8):e1003539
PLoS pathogens 2013;9(8):e1003539
Non-canonical inflammasome activation targets caspase-11.
Kayagaki N, Warming S, Lamkanfi M, Vande Walle L, Louie S, Dong J, Newton K, Qu Y, Liu J, Heldens S, Zhang J, Lee WP, Roose-Girma M, Dixit VM
Nature 2011 Oct 16;479(7371):117-21
Nature 2011 Oct 16;479(7371):117-21
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Untreated (¡V) and treated (+) Raw264.7 whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with IL1 beta antibody (GTX74034) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Untreated (¡V) and treated (+) Raw264.7 whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with IL1 beta antibody (GTX74034) diluted at 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- An ELISA plate is coated with 50 ?L of IL1 beta recombinant protein at concentration of 5 ?g/mL. The coated protein is detected with anti-IL1 beta antibody (GTX74034) at concentration ranged from 0.01 to 0.15 ?g/mL.
