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LearnNB600-633
antibody from Novus Biologicals
Targeting: IL1B
IL-1B, IL1-BETA, IL1F2
Western blot
ELISA Immunocytochemistry Immunoprecipitation Immunohistochemistry Immunoelectron microscopyAntibody data
- Antibody Data
- Antigen structure
- References [27]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [1]
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- Product number
- NB600-633 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-633, RRID:AB_10001060
- Product name
- Rabbit Polyclonal IL-1 beta/IL-1F2 Antibody
- Antibody type
- Polyclonal
- Description
- IgG purified. Detects IL-1 beta and does not cross react with IL-1 alpha. Weakly detects the non-denatured 31 kDa precursor molecule but strongly detects all of the 17 kDa mature molecule. Additionally this IL-1 beta antibody will bind the denatured 31 kDa precursor molecule with greater affinity than the native form, but will often detect past the 35 kDa mark. This IL-1 beta antibody has also been shown to be useful in neutralization of human and primate IL-1 beta activity in bioassays.
- Reactivity
- Human, Mouse, Rat, Bacteria, Canine, Porcine, Simian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 2 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references cAMP signaling primes lung endothelial cells to activate caspase-1 during Pseudomonas aeruginosa infection.
Macrophage miR-34a Is a Key Regulator of Cholesterol Efflux and Atherosclerosis.
Pharmacological Transection of Brain-Spinal Cord Communication Blocks Pain-Induced Hemorrhage and Locomotor Deficits after Spinal Cord Injury in Rats.
Eplerenone Ameliorates Cell Pyroptosis in Contralateral Kidneys of Rats with Unilateral Ureteral Obstruction.
p16(Ink4a) deletion in cells of the intervertebral disc affects their matrix homeostasis and senescence associated secretory phenotype without altering onset of senescence.
Prostaglandin-mediated effects in early canine corpus luteum: In vivo effects on vascular and immune factors.
PAI-1 augments mucosal damage in colitis.
Penehyclidine hydrochloride suppressed peripheral nerve injury-induced neuropathic pain by inhibiting microglial MAPK/p-p38/IL-1β pathway activation.
Towards A Microbead Occlusion Model of Glaucoma for a Non-Human Primate.
Placental inflammation in pre-eclampsia by Nod-like receptor protein (NLRP)3 inflammasome activation in trophoblasts.
Intranuclear delivery of synthetic nuclear factor-kappa B p65 reduces inflammasomes after surgery.
Transgenic mice overexpressing human TNF-α experience early onset spontaneous intervertebral disc herniation in the absence of overt degeneration.
The human heart contains distinct macrophage subsets with divergent origins and functions.
Role of Neuron-Glial Interaction Mediated by IL-1β in Ectopic Tooth Pain.
Porphyromonas gingivalis-Derived Lipopolysaccharide Combines Hypoxia to Induce Caspase-1 Activation in Periodontitis.
Methylene Blue Mitigates Acute Neuroinflammation after Spinal Cord Injury through Inhibiting NLRP3 Inflammasome Activation in Microglia.
A novel intrinsically disordered outer membrane lipoprotein of Aggregatibacter actinomycetemcomitans binds various cytokines and plays a role in biofilm response to interleukin-1β and interleukin-8.
Interaction between inflammatory mediators and miRNAs in Helicobacter pylori infection.
Phosphodiesterase 3B (PDE3B) regulates NLRP3 inflammasome in adipose tissue.
Neuronal tumour necrosis factor-α and interleukin-1β expression in a porcine model of intracerebral haemorrhage: Modulation by U-74389G.
Interleukin-1β/Iinterleukin-1 receptor-associated kinase 1 inflammatory signaling contributes to persistent Gankyrin activation during hepatocarcinogenesis.
Identification of a novel bacterial outer membrane interleukin-1Β-binding protein from Aggregatibacter actinomycetemcomitans.
Obesity promotes breast cancer by CCL2-mediated macrophage recruitment and angiogenesis.
Interleukin-1β is internalised by viable Aggregatibacter actinomycetemcomitans biofilm and locates to the outer edges of nucleoids.
Phagocytosis of cells dying through autophagy induces inflammasome activation and IL-1β release in human macrophages.
Phagocytosis of cells dying through autophagy induces inflammasome activation and IL-1β release in human macrophages.
Trimeric form of intracellular ATP synthase subunit β of Aggregatibacter actinomycetemcomitans binds human interleukin-1β.
Renema P, Hardy KS, Housley N, Dunbar G, Annamdevula N, Britain A, Spadafora D, Leavesley S, Rich T, Audia JP, Alvarez DF
American journal of physiology. Lung cellular and molecular physiology 2020 May 1;318(5):L1074-L1083
American journal of physiology. Lung cellular and molecular physiology 2020 May 1;318(5):L1074-L1083
Macrophage miR-34a Is a Key Regulator of Cholesterol Efflux and Atherosclerosis.
Xu Y, Xu Y, Zhu Y, Sun H, Juguilon C, Li F, Fan D, Yin L, Zhang Y
Molecular therapy : the journal of the American Society of Gene Therapy 2020 Jan 8;28(1):202-216
Molecular therapy : the journal of the American Society of Gene Therapy 2020 Jan 8;28(1):202-216
Pharmacological Transection of Brain-Spinal Cord Communication Blocks Pain-Induced Hemorrhage and Locomotor Deficits after Spinal Cord Injury in Rats.
Davis JA, Bopp AC, Henwood MK, Baine RE, Cox CC, Grau JW
Journal of neurotrauma 2020 Aug 1;37(15):1729-1739
Journal of neurotrauma 2020 Aug 1;37(15):1729-1739
Eplerenone Ameliorates Cell Pyroptosis in Contralateral Kidneys of Rats with Unilateral Ureteral Obstruction.
Ma X, Chang Y, Xiong Y, Wang Z, Wang X, Xu Q
Nephron 2019;142(3):233-242
Nephron 2019;142(3):233-242
p16(Ink4a) deletion in cells of the intervertebral disc affects their matrix homeostasis and senescence associated secretory phenotype without altering onset of senescence.
Novais EJ, Diekman BO, Shapiro IM, Risbud MV
Matrix biology : journal of the International Society for Matrix Biology 2019 Sep;82:54-70
Matrix biology : journal of the International Society for Matrix Biology 2019 Sep;82:54-70
Prostaglandin-mediated effects in early canine corpus luteum: In vivo effects on vascular and immune factors.
Tavares Pereira M, Gram A, Nowaczyk R, Boos A, Hoffmann B, Janowski T, Kowalewski MP
Reproductive biology 2019 Mar;19(1):100-111
Reproductive biology 2019 Mar;19(1):100-111
PAI-1 augments mucosal damage in colitis.
Kaiko GE, Chen F, Lai CW, Chiang IL, Perrigoue J, Stojmirović A, Li K, Muegge BD, Jain U, VanDussen KL, Goggins BJ, Keely S, Weaver J, Foster PS, Lawrence DA, Liu TC, Stappenbeck TS
Science translational medicine 2019 Mar 6;11(482)
Science translational medicine 2019 Mar 6;11(482)
Penehyclidine hydrochloride suppressed peripheral nerve injury-induced neuropathic pain by inhibiting microglial MAPK/p-p38/IL-1β pathway activation.
Zheng SH, Yan CY, Duan N, Wang W, Mei XP
Molecular pain 2019 Jan-Dec;15:1744806919858260
Molecular pain 2019 Jan-Dec;15:1744806919858260
Towards A Microbead Occlusion Model of Glaucoma for a Non-Human Primate.
Lambert WS, Carlson BJ, Ghose P, Vest VD, Yao V, Calkins DJ
Scientific reports 2019 Aug 9;9(1):11572
Scientific reports 2019 Aug 9;9(1):11572
Placental inflammation in pre-eclampsia by Nod-like receptor protein (NLRP)3 inflammasome activation in trophoblasts.
Stødle GS, Silva GB, Tangerås LH, Gierman LM, Nervik I, Dahlberg UE, Sun C, Aune MH, Thomsen LCV, Bjørge L, Iversen AC
Clinical and experimental immunology 2018 Jul;193(1):84-94
Clinical and experimental immunology 2018 Jul;193(1):84-94
Intranuclear delivery of synthetic nuclear factor-kappa B p65 reduces inflammasomes after surgery.
Cheon SY, Kim JM, Kim EJ, Kim SY, Kam EH, Ho CC, Lee SK, Koo BN
Biochemical pharmacology 2018 Dec;158:141-152
Biochemical pharmacology 2018 Dec;158:141-152
Transgenic mice overexpressing human TNF-α experience early onset spontaneous intervertebral disc herniation in the absence of overt degeneration.
Gorth DJ, Shapiro IM, Risbud MV
Cell death & disease 2018 Dec 18;10(1):7
Cell death & disease 2018 Dec 18;10(1):7
The human heart contains distinct macrophage subsets with divergent origins and functions.
Bajpai G, Schneider C, Wong N, Bredemeyer A, Hulsmans M, Nahrendorf M, Epelman S, Kreisel D, Liu Y, Itoh A, Shankar TS, Selzman CH, Drakos SG, Lavine KJ
Nature medicine 2018 Aug;24(8):1234-1245
Nature medicine 2018 Aug;24(8):1234-1245
Role of Neuron-Glial Interaction Mediated by IL-1β in Ectopic Tooth Pain.
Komiya H, Shimizu K, Noma N, Tsuboi Y, Honda K, Kanno K, Ohara K, Shinoda M, Ogiso B, Iwata K
Journal of dental research 2018 Apr;97(4):467-475
Journal of dental research 2018 Apr;97(4):467-475
Porphyromonas gingivalis-Derived Lipopolysaccharide Combines Hypoxia to Induce Caspase-1 Activation in Periodontitis.
Cheng R, Liu W, Zhang R, Feng Y, Bhowmick NA, Hu T
Frontiers in cellular and infection microbiology 2017;7:474
Frontiers in cellular and infection microbiology 2017;7:474
Methylene Blue Mitigates Acute Neuroinflammation after Spinal Cord Injury through Inhibiting NLRP3 Inflammasome Activation in Microglia.
Lin ZH, Wang SY, Chen LL, Zhuang JY, Ke QF, Xiao DR, Lin WP
Frontiers in cellular neuroscience 2017;11:391
Frontiers in cellular neuroscience 2017;11:391
A novel intrinsically disordered outer membrane lipoprotein of Aggregatibacter actinomycetemcomitans binds various cytokines and plays a role in biofilm response to interleukin-1β and interleukin-8.
Ahlstrand T, Tuominen H, Beklen A, Torittu A, Oscarsson J, Sormunen R, Pöllänen MT, Permi P, Ihalin R
Virulence 2017 Feb 17;8(2):115-134
Virulence 2017 Feb 17;8(2):115-134
Interaction between inflammatory mediators and miRNAs in Helicobacter pylori infection.
Rossi AF, Cadamuro AC, Biselli-Périco JM, Leite KR, Severino FE, Reis PP, Cordeiro JA, Silva AE
Cellular microbiology 2016 Oct;18(10):1444-58
Cellular microbiology 2016 Oct;18(10):1444-58
Phosphodiesterase 3B (PDE3B) regulates NLRP3 inflammasome in adipose tissue.
Ahmad F, Chung YW, Tang Y, Hockman SC, Liu S, Khan Y, Huo K, Billings E, Amar MJ, Remaley AT, Manganiello VC
Scientific reports 2016 Jun 20;6:28056
Scientific reports 2016 Jun 20;6:28056
Neuronal tumour necrosis factor-α and interleukin-1β expression in a porcine model of intracerebral haemorrhage: Modulation by U-74389G.
Bimpis A, Papalois A, Voumvourakis K, Oláh O, Tiszlavicz L, Liapi C
Brain research 2015 Jul 30;1615:98-105
Brain research 2015 Jul 30;1615:98-105
Interleukin-1β/Iinterleukin-1 receptor-associated kinase 1 inflammatory signaling contributes to persistent Gankyrin activation during hepatocarcinogenesis.
Su B, Luo T, Zhu J, Fu J, Zhao X, Chen L, Zhang H, Ren Y, Yu L, Yang X, Wu M, Feng G, Li S, Chen Y, Wang H
Hepatology (Baltimore, Md.) 2015 Feb;61(2):585-97
Hepatology (Baltimore, Md.) 2015 Feb;61(2):585-97
Identification of a novel bacterial outer membrane interleukin-1Β-binding protein from Aggregatibacter actinomycetemcomitans.
Paino A, Ahlstrand T, Nuutila J, Navickaite I, Lahti M, Tuominen H, Välimaa H, Lamminmäki U, Pöllänen MT, Ihalin R
PloS one 2013;8(7):e70509
PloS one 2013;8(7):e70509
Obesity promotes breast cancer by CCL2-mediated macrophage recruitment and angiogenesis.
Arendt LM, McCready J, Keller PJ, Baker DD, Naber SP, Seewaldt V, Kuperwasser C
Cancer research 2013 Oct 1;73(19):6080-93
Cancer research 2013 Oct 1;73(19):6080-93
Interleukin-1β is internalised by viable Aggregatibacter actinomycetemcomitans biofilm and locates to the outer edges of nucleoids.
Paino A, Lohermaa E, Sormunen R, Tuominen H, Korhonen J, Pöllänen MT, Ihalin R
Cytokine 2012 Nov;60(2):565-74
Cytokine 2012 Nov;60(2):565-74
Phagocytosis of cells dying through autophagy induces inflammasome activation and IL-1β release in human macrophages.
Petrovski G, Ayna G, Majai G, Hodrea J, Benko S, Mádi A, Fésüs L
Autophagy 2011 Mar;7(3):321-30
Autophagy 2011 Mar;7(3):321-30
Phagocytosis of cells dying through autophagy induces inflammasome activation and IL-1β release in human macrophages.
Petrovski G, Ayna G, Majai G, Hodrea J, Benko S, Mádi A, Fésüs L
Autophagy 2011 Mar;7(3):321-30
Autophagy 2011 Mar;7(3):321-30
Trimeric form of intracellular ATP synthase subunit β of Aggregatibacter actinomycetemcomitans binds human interleukin-1β.
Paino A, Tuominen H, Jääskeläinen M, Alanko J, Nuutila J, Asikainen SE, Pelliniemi LJ, Pöllänen MT, Chen C, Ihalin R
PloS one 2011 Apr 18;6(4):e18929
PloS one 2011 Apr 18;6(4):e18929
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IL-1 beta/IL-1F2 Antibody [NB600-633] - Antibody was used at a 1:200 dilution incubated 1h at room temperature to detect dog IL-1b by Western blot. Lane 1 and 2 were loaded with 2.5 ug and 500 ng of dog IL-1b respectively. The molecular weight of the detected band is estimated by comparison to molecular weight markers (not shown). Detection occurred using a 1:3,000 dilution of IRDye (TM) 800 conjugated Donkey anti-Rabbit IgG for 1h at room temperature.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IL-1 beta/IL-1F2 Antibody [NB600-633] - Analysis of IL-1 beta in Rhesus fetal membrane using anti-IL-1 beta antibody. Image from verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IL-1 beta/IL-1F2 Antibody [NB600-633] - pro-IL-1beta in glioblastoma cells. Image from verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IL-1 beta/IL-1F2 Antibody [NB600-633] - Lane 1: Human IL-1Beta. Load: 5 ng per lane. Primary antibody: Human IL-1Beta antibody at 1:2,000 for overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT. Predicted/Observed size: 17 kDa, 17 kDa for Human IL-1Beta. Other band(s): Unspecific band at 35 kDa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: IL-1 beta/IL-1F2 Antibody [NB600-633] - Analysis of: Human IL1beta antibody Secondary antibody: Peroxidase goat anti-rabbit at 1:10,000 for 45 min at RT Localization: cytoplasm Staining: Close up of medullary lymph node: positive staining in the cytoplasm of circulating macrophages. Neg Ctr (far right) normal rabbit IgG with pH 6.2 40X
