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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [6]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- MAB6964 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-1 beta/IL-1F2 Propeptide Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human IL-1 beta/IL-1F2 Propeptide in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-1 alpha, rhIL-1F7, rhIL-1F10, rhIL-18, rhIL-36 alpha, rhIL-36 beta, IL-36 gamma, rhIL-36Ra, recombinant mouse IL-1Ra, or the mature peptides of recombinant IL-1 beta from canine, cotton rat, equine, feline, guinea pig, human, mouse, porcine, rabbit, rat, or rhesus is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P01584- Isotype
- IgG
- Antibody clone number
- 615417
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Levistilide A Ameliorates NLRP3 Expression Involving the Syk-p38/JNK Pathway and Peripheral Obliterans in Rats.
MicroRNA-9 Inhibits NLRP3 Inflammasome Activation in Human Atherosclerosis Inflammation Cell Models through the JAK1/STAT Signaling Pathway.
Azithromycin decreases NALP3 mRNA stability in monocytes to limit inflammasome-dependent inflammation.
NLRP3 inflammasome: a novel link between lipoproteins and atherosclerosis.
Propionibacterium acnes Induces IL-1β secretion via the NLRP3 inflammasome in human monocytes.
Unsaturated fatty acids prevent activation of NLRP3 inflammasome in human monocytes/macrophages.
Guo H, Sun L, Ling S, Xu JW
Mediators of inflammation 2018;2018:7304096
Mediators of inflammation 2018;2018:7304096
MicroRNA-9 Inhibits NLRP3 Inflammasome Activation in Human Atherosclerosis Inflammation Cell Models through the JAK1/STAT Signaling Pathway.
Wang Y, Han Z, Fan Y, Zhang J, Chen K, Gao L, Zeng H, Cao J, Wang C
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2017;41(4):1555-1571
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2017;41(4):1555-1571
Azithromycin decreases NALP3 mRNA stability in monocytes to limit inflammasome-dependent inflammation.
Lendermon EA, Coon TA, Bednash JS, Weathington NM, McDyer JF, Mallampalli RK
Respiratory research 2017 Jun 28;18(1):131
Respiratory research 2017 Jun 28;18(1):131
NLRP3 inflammasome: a novel link between lipoproteins and atherosclerosis.
Li WL, Hua LG, Qu P, Yan WH, Ming C, Jun YD, Yuan LD, Nan N
Archives of medical science : AMS 2016 Oct 1;12(5):950-958
Archives of medical science : AMS 2016 Oct 1;12(5):950-958
Propionibacterium acnes Induces IL-1β secretion via the NLRP3 inflammasome in human monocytes.
Qin M, Pirouz A, Kim MH, Krutzik SR, Garbán HJ, Kim J
The Journal of investigative dermatology 2014 Feb;134(2):381-388
The Journal of investigative dermatology 2014 Feb;134(2):381-388
Unsaturated fatty acids prevent activation of NLRP3 inflammasome in human monocytes/macrophages.
L'homme L, Esser N, Riva L, Scheen A, Paquot N, Piette J, Legrand-Poels S
Journal of lipid research 2013 Nov;54(11):2998-3008
Journal of lipid research 2013 Nov;54(11):2998-3008
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human IL-1 beta/IL-1F2 Propeptide by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 80 nM PMA for 48 hours. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IL-1 beta/IL-1F2 Propeptide Monoclonal Antibody (Catalog # MAB6964) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL-1 beta/IL-1F2 Propeptide at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human IL-1 beta/IL-1F2 Propeptide by Simple WesternTM. Simple Western lane view shows lysates of THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with PMA and LPS, loaded at 0.2 mg/mL. A specific band was detected for IL-1 beta/IL-1F2 Propeptide at approximately 40 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human IL-1 beta/IL-1F2 Propeptide Monoclonal Antibody (Catalog # MAB6964). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-1 beta/IL-1F2 in Human PBMCs. IL-1 beta/IL-1F2 was detected in immersion fixed human peripheral blood mononuclear cells using Mouse Anti-Human IL-1 beta/IL-1F2 Propeptide Monoclonal Antibody (Catalog # MAB6964) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI(blue). Specific staining was localized to cytoplasmic. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
