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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- TA323422 - Provider product page
- Provider
- OriGene
- Product name
- Anti-SIRT3 Rabbit Polyclonal Antibody
- Antibody type
- Polyclonal
- Description
- Anti-SIRT3 Rabbit Polyclonal Antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- SIRT3
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µl
- Concentration
- 1mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Predicted band size: 44 kDa . Positive control: Mouse brain and human liver cancer tissue lysate. Recommended dilution: 1/500-2000. (Gel: 12%SDS-PAGE Lane1: Mouse brain tissue lysate Lane2: Human liver cancer tissue lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 90 seconds)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Predicted cell location: Cytoplasm. Positive control: Human brain tissue. Recommended dilution: 1/15-50 The image on the left is immunohistochemistry of paraffin-embedded human brain tissue using SIRT3 antibody at dilution 1/30, on the right is treated with the fusion protein. (Original magnification: x200)
- Validation comment
- IHC
