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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- ELISA [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- NBP2-52563 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Sirtuin 3/SIRT3 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Sirtuin 3/SIRT3 Antibody (6B2A1) [NBP2-52563] - Analysis using SIRT3 mAb against HEK293 (1) and SIRT3 (AA: 155-290)-hIgGFc transfected HEK293 (2) cell lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Sirtuin 3/SIRT3 Antibody (6B2A1) [NBP2-52563] - Analysis using SIRT3 mAb against human SIRT3 (AA: 155-290) recombinant protein. (Expected MW is 41.3 kDa)
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Sirtuin 3/SIRT3 Antibody (6B2A1) [NBP2-52563] - Analysis using SIRT3 mouse mAb against PANC-1 (1) cell lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Sirtuin 3/SIRT3 Antibody (6B2A1) [NBP2-52563] - Black line: Control Antigen (100 ng); Purple line: Antigen(10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry Whole-Mount: Sirtuin 3/SIRT3 Antibody (6B2A1) [NBP2-52563] - Cochlear whole mount from wild-type and Sirt3-KO mice, immunolabeled with MYO7, SIRT3, and DAPI. The cross-hairs show the position from which the side view is taken. SIRT3 immunoreactivity only is shown in the right panels. IHC-WhMt image submitted by a verified customer review.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Sirtuin 3/SIRT3 Antibody (6B2A1) [NBP2-52563] - Analysis of HeLa cells using SIRT3 mouse mAb (green) and negative control (red).
