H00023410-M09

antibody from Abnova Corporation

Targeting: SIRT3 SIR2L3

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00023410-M09

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00023410-M09, RRID:AB_1580642

Product name

SIRT3 monoclonal antibody (M09), clone 1A4

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant SIRT3.

Antigen sequence

PLPQRFLLHVVDFPMADLLLILGTSLEVEPFASLT
EAVRSSVPRLLINRDLVGPLAWHPRSRDVAQLGDV
VHGVESLVELLGWTEEMRDLVQRETGKLDGPDK


Isotype

IgG

Antibody clone number

1A4

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of SIRT3 expression in transfected 293T cell line by SIRT3 monoclonal antibody (M09), clone 1A4.Lane 1: SIRT3 transfected lysate (Predicted MW: 28.6 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged SIRT3 is 3 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol