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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
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- Product number
- PAB6891 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB6891, RRID:AB_1580641
- Product name
- SIRT3 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of SIRT3.
- Antigen sequence
C-QRETGKLDGPDK- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references SIRT3, a mitochondrial sirtuin deacetylase, regulates mitochondrial function and thermogenesis in brown adipocytes.
Shi T, Wang F, Stieren E, Tong Q
The Journal of biological chemistry 2005 Apr 8;280(14):13560-7
The Journal of biological chemistry 2005 Apr 8;280(14):13560-7
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- SIRT3 polyclonal antibody (Cat # PAB6891) (0.2 ug/mL) staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- HEK293 overexpressing SIRT3 and probed with SIRT3 polyclonal antibody (Cat # PAB6891) (mock transfection in first lane), tested by Origene.
