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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [3]
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- Product number
- BML-SA463-0100 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#BML-SA463-0100, RRID:AB_2052556
- Product name
- SIRT3 polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full length protein
- Reactivity
- Human, Rat, Bovine
- Host
- Rabbit
- Vial size
- 100 μl
- Storage
- -20°C
- Handling
- Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20¡C.
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Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Western blot analysis of recombinant human sirtuins, cell and tissue extracts. Blots were probed with SIRT3, pAb at 1/1000. Secondary antibodies were coupled to alkaline phosphatase and developed with BCIP/NBT . Arrows indicate positions of precursor (P) and mature (M) forms of SIRT3. Numbers indicate molecular weights of marker proteins in kDa. Lanes: M-MW markers, B-bovine heart, R-rat thymus, S3-recomb. SIRT3, 50 ng, L-HL60 cells, Hn-HeLa nucleus, S5-recomb. SIRT5, 50 ng, J-Jurkat cells, Hc-HeLa cytosol, and S2-recomb. SIRT2, 50 ng.
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Western blot analysis of recombinant human sirtuins, cell and tissue extracts. Blots were probed with SIRT3, pAb at 1/1000. Secondary antibodies were coupled to horseradish peroxidase and developed by ECL. Arrows indicate positions of precursor (P) and mature (M) forms of SIRT3. Numbers indicate molecular weights of marker proteins in kDa. Lanes: M-MW markers, L-HL60 cells, and J-Jurkat cells.
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Immunohistochemistry staining of formalin fixed, paraffin-embedded human liver tumor tissue using SIRT3 antibody (Prod. no. BML-SA463) showing cell membrane staining of hepatocytes.
