Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [4]
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Validation data
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- Product number
- NBP1-90107 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-90107, RRID:AB_11031663
- Product name
- Rabbit Polyclonal HA-1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human HA-1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references The human minor histocompatibility antigen 1 is a RhoGAP.
de Kreuk BJ, Schaefer A, Anthony EC, Tol S, Fernandez-Borja M, Geerts D, Pool J, Hambach L, Goulmy E, Hordijk PL
PloS one 2013;8(9):e73962
PloS one 2013;8(9):e73962
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HA-1 Antibody [NBP1-90107] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HA-1 Antibody [NBP1-90107] - Analysis in human cell line MOLT-4.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HA-1 Antibody [NBP1-90107] - Staining of human appendix shows strong cytoplasmic positivity in lymphoid tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HA-1 Antibody [NBP1-90107] - Staining of human cerebral cortex shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HA-1 Antibody [NBP1-90107] - Staining of human spleen shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HA-1 Antibody [NBP1-90107] - Staining in human spleen and cerebral cortex tissues using anti-ARHGAP45 antibody. Corresponding ARHGAP45 RNA-seq data are presented for the same tissues.