Systems biology, functional genomics and high-throughput proteomics efforts have massively expedited gene-product annotation and characterization. However, identifying affinity reagents suitable for secondary analyses of proteins can pose a challenge. While antibodies are widely used, their efficacy in different biological systems and experimental applications often varies depending on their immunogen or the organism in which they were produced. In fact, the likelihood that an antibody will function in a novel application or cell/ tissue type depends heavily on its affinity for the antigen and the type of epitope recognized, as well as on the antigen's concentration, folding status and post-translational modifications. Without detailed understanding of the properties of both the antibody and the biological system in which it will be used, antibody selection can require guesswork.
Antibodypedia is a searchable database of antibodies against human proteins. It aims to provide the research community with information on the effectiveness of specific antibodies in specific applications—to help scientists select the right antibody for the right application.
The Antibodypedia database was originally developed within the 6th framework EU program Proteome Binders and the project is part of the Human Antibody Initiative.
Antibodypedia contains information about publicly available antibodies generated by academic or commercial providers and directed against human protein targets; we hope to extend coverage to a range of model organisms in the near future. The database is organized in a ‘gene-centric’ manner to provide users with an overview of all antibodies available against a particular target. All antibody pages link directly to the provider for ease of access and use of the database is free-of-charge.
We encourage commercial providers to submit information regarding their inventory of antibodies along with links to quality control data. Independent users can submit their own application-specific experimental data using standard validation criteria (supportive or non-supportive) developed with the assistance of an international advisory board recruited from academic research institutions. Users can also comment on specific antibodies without submitting validation data.
Antibodies can at any time be withdrawn from the database by the provider and all data (from provider and users) will in this case be removed.