Searching Antibodypedia

Antibodypedia is a searchable database of publicly available antibodies generated by commercial or academic providers. Its organization is gene-centric: in other words, it is structured such that all antibodies deposited in the database and directed against a particular protein target are accessible from within a page devoted to that gene product.

Recent changes

Version 7 of Antibodypedia includes a redesigned search function. Whereas before it was possible to define stringent search parameters within 'Advanced Search', much of the functionality of 'Advanced Search' has now been transferred to a series of filters on search results pages. 

  • It is still possible to retrieve a list of all gene-products in Antibodypedia (along with associated antibodies, if applicable) by clicking the 'Explore' tab when no text has been entered into the search box. 
  • Searches for all gene-products encoded on a particular (human) chromosome or all gene-products within a specific protein family (such as transcription factors) can still be performed by selecting those options from the 'Field' menu in Advanced Search when no text has been entered into the search box. 
  • Searches for gene-products with redundant names can be performed by entering their information into the search box and selecting AND as well as any relevant gene-specific attributes from the 'Field' menu. 

Identifying antibodies

Due to Antibodypedia's antigen-centric structure, the results of antibody searches are organized by target protein. The results of a parameter-based search (such as protein_family:"Transcription factors") are displayed alphabetically by antigen. Free-text search results are returned by relevance, taking into account names, synonyms and descriptions of genes encoding the queried antigen, along with other terms, to determine the position in which an antigen result is displayed.

  • A dedicated page now lists possible target antigens retrieved by a query, as well as the number of antibodies held by Antibodypedia that detect each target. Clicking on the antigen of interest will load a separate page cataloguing antibodies reactive against it. 
  • This antibody results page will list all antibodies in the database capable of detecting the antigen of interest. Antibody results are grouped by provider and rank-ordered according to the quantity and nature of the data available on their use. 
    • Antibody search results can be refined by the application of filters found on the left side of the antibody results page. 
    • The antibody attributes displayed on this page can be altered by selecting from the options within 'Show additional columns'. 
    • Checkboxes to the left of each antibody listing permit cross-comparison of their attributes.
    • Antibodies can also be investigated in depth by clicking within each listing; this will load a dedicated page detailing the reagent's reactivity, purchase information and any associated data and references.

The Antibodypedia ranking algorithm

Provider rankings on antibody search results pages are determined according to the score of their best antibody. If two independent providers list antibodies with identical scores, the score of the second-most-efficacious antibody is taken into account, and so on, until rank is established (similar to the 'Alternative vote' system). Complete information on antibody score determination can be found in our 'Antibody scoring' section.

Filtering search results

Antibody search results can now be filtered by application, presence of references, provider, host, reactivity and antibody type/ clonality. OR searches are possible for all categories but references ('yes' or 'no'). 

Please note that antibodies recommended for RNA IP are listed under 'Immunoprecipitation'.