Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [5]
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Validation data
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- Product number
- MA5-24635 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CARS Monoclonal Antibody (CL2304)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: SQCNLYMAAR KAVRKRPNQA LLENIALYLT HMLKIFGAVE EDSSLGFPVG GPGTSLSLEA TVMPYLQVLS EFREGVRKIA REQKVPEILQ LSDALRDNIL PELGVRFEDH EGLPTVVKLV DRNTLLKERE EKRRVE Highest antigen sequence identity to the following orthologs: Mouse - 86%, Rat - 86%. Binds to an epitope located within the peptide sequence EDSSLGFPVG as determined by overlapping synthetic peptides.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- CL2304
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CARS in Lane1: Marker (kDa), Lane 2: Human cell line U-251 MG. Samples were probed using a CARS Monoclonal Antibody (Product # MA5-24635).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CARS in Lane1: Marker (kDa), Lane 2: Human cell line HeLa cytoplasmic fraction, Lane 3: Human cell line HeLa membrane fraction, Lane 4: Human cell line HeLa nuclear fraction, Lane 5: Human cell line HeLa chromatin fraction, Lane 6: Human cell line HeLa cytoskeletal fraction. Samples were probed using a CARS Monoclonal Antibody (Product # MA5-24635).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of CARS in human placenta shows cytoplasmic positivity in the trophoblast. Samples were probed using a CARS Monoclonal Antibody (Product # MA5-24635).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of CARS in human skin shows strong cytoplasmic positivty in epidermis cells. Samples were probed using a CARS Monoclonal Antibody (Product # MA5-24635).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of CARS in human breast cancer tissue shows cytoplasmic immunoreactivity in tumor cells. Samples were probed using a CARS Monoclonal Antibody (Product # MA5-24635).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of CARS in human colon tissue shows strong cytoplasmic immunoreactivity in glandular epithelium cells. Samples were probed using a CARS Monoclonal Antibody (Product # MA5-24635).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of CARS in human kidney tissue shows strong cytoplasmic immunoreactivity in renal tubules. Samples were probed using a CARS Monoclonal Antibody (Product # MA5-24635).