PAB21491

antibody from Abnova Corporation

Targeting: POLR1F A43, RPA43, TWISTNB

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB21491

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB21491, RRID:AB_10962066

Product name

TWISTNB polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant TWISTNB.

Antigen sequence

QTMEINMGDELEFEVFRLDSDAAGVFCIRGKLNIT
SLQFKRSEVSEEVTENGTEEAAKKPKKKKKKKDPE
TYEVDSGTTKLAD


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate).Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells with TWISTNB polyclonal antibody (Cat # PAB21491).

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 with TWISTNB polyclonal antibody (Cat # PAB21491) at 1-4 ug/mL dilution shows positivity in nucleus and nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebellum with TWISTNB polyclonal antibody (Cat # PAB21491) shows strong cytoplasmic positivity in Purkinje cells at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)