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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-21361 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ATP5D Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HepG2, Molt-4, Raji, mouse brain. Predicted reactivity: Mouse (88%), Rat (89%), Rhesus Monkey (98%), Bovine (93%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.0 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ATP synthase H+ transporting mitochondrial F1 complex delta subunit precur using 30 µg of A) HepG2 (B) MOLT4 and C) Raji lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with an ATP synthase H+ transporting mitochondrial F1 complex delta subunit precur polyclonal antibody (Product # PA5-21361) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ATP5D Polyclonal Antibody (Product # PA5-21361). Sample (50 µg of whole cell lysate). Lane A: Mouse brain. 12% SDS PAGE. ATP5D Polyclonal Antibody (Product # PA5-21361) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ATP5D Polyclonal Antibody (Product # PA5-21361). HepG2 and mitochondria extracts (30 µg) were separated by SDS-PAGE, and the membrane was blotted with ATP5D Polyclonal Antibody (Product # PA5-21361) diluted at 1:2,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ATP synthase delta antibody detects ATP5D protein at mitochondria on human breast cancer by immunohistochemical analysis. Sample: Paraffin-embedded breast cancer. ATP synthase delta antibody (Product # PA5-21361) dilution: 1:250. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
