MA5-13078

antibody from Invitrogen Antibodies

Targeting: ACP3 ACP-3, ACPP, PAP, TM-PAP

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-13078

Provider

Invitrogen Antibodies

Product name

Prostate Specific Acid Phosphatase Monoclonal Antibody (PASE/4LJ)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-13078 targets Prostate Specific Acid Phosphatase in IF and IHC (P) applications and shows reactivity with Human samples. The MA5-13078 immunogen is purified prostatic acid phosphatase (PSAP) from human seminal plasma.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

PASE/4LJ

Vial size

500 µL

Concentration

0.2 mg/mL

Storage

4° C

References

Submitted references

A comparison of prostatic development in xenografts of human fetal prostate and human female fetal proximal urethra grown in dihydrotestosterone-treated hosts.

Cunha GR, Cao M, Franco O, Baskin LS
Differentiation; research in biological diversity 2020 Sep-Oct;115:37-52

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Thyroid transcription factor-1 may be expressed in ductal adenocarcinoma of the prostate:a potential pitfall.

Lim TK, Teo C, Giron DM, Chong YL, Cheng C, Tan PH
Journal of clinical pathology 2007 Aug;60(8):941-3

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Metastasizing seminal vesicle adenocarcinoma in a Wistar rat.

Teredesai A, Wöhrmann T
Journal of veterinary medicine. A, Physiology, pathology, clinical medicine 2005 Apr;52(3):131-4

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of Prostate Specific Acid Phosphatase Antibody (PASE/4LJ) was performed using 70% confluent log phase LNCaP cells. The cells were fixed with methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Prostate Specific Acid Phosphatase Antibody (PASE/4LJ) Mouse Monoclonal Antibody (Product # MA5-13078) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Cytoskeleton was stained with alpha-Tubulin Monoclonal Antibody (Product # PA5-16891, 1 µg/mL) followed by Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 594 conjugate (Product # A-11037) (Panel c: red). Panel d represents the merged image showing membranous localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Formalin-fixed, paraffin-embedded human prostate stained with Prostate Specific Acid Phosphatase antibody using alkaline phosphatase-conjugate and fast red chromogen. Note intense cytoplasmic staining of glandular epithelia