PA5-22154

antibody from Invitrogen Antibodies

Targeting: BABAM2 BRCC4, BRCC45, BRE

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-22154

Provider

Invitrogen Antibodies

Product name

Anti-BRE Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

PA5-22154 targets BRE in ICC, IF, IHC (P), and WB applications and shows reactivity with Human and mouse samples. The PA5-22154 immunogen is recombinant protein fragment contain a sequence corresponding to a region within amino acids 51 and 229 (Q9NXR7) of BRE.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/ml

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of BRE using A) 30 µg A431 whole cell lysate and B) 30 µg HeLa whole cell lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a BRE polyclonal antibody (Product # PA5-22154) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of BRE using A) 30 µg Neuro2A whole cell lysate (B) 30 µg GL261 whole cell lysate and C) 30 µg C8D30 whole cell lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a BRE polyclonal antibody (Product # PA5-22154) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of BRE using A) 30 µg PC-12 whole cell lysate and B) 30 µg Rat2 whole cell lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a BRE polyclonal antibody (Product # PA5-22154) at a dilution of 1:1000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of BRE in paraformaldehyde-fixed HeLa cells using a BRE polyclonal antibody (Product # PA5-22154) at a 1:200 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of BRE in the nucleus of paraffin-embedded Cal27 xenograft using a BRE polyclonal antibody (Product # PA5-22154) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of BRE in the nucleus and cytosol of paraffin-embedded mouse pancreas tissue using a BRE polyclonal antibody (Product # PA5-22154) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of BRE in the nucleus and cytosol of paraffin-embedded mouse colon tissue using a BRE polyclonal antibody (Product # PA5-22154) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of BRE in the nucleus and cytosol of paraffin-embedded rat middle brain tissue using a BRE polyclonal antibody (Product # PA5-22154) at a dilution of 1:500.