Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
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- Product number
- PA5-22154 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-BRE Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- PA5-22154 targets BRE in ICC, IF, IHC (P), and WB applications and shows reactivity with Human and mouse samples. The PA5-22154 immunogen is recombinant protein fragment contain a sequence corresponding to a region within amino acids 51 and 229 (Q9NXR7) of BRE.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/ml
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BRE using A) 30 µg A431 whole cell lysate and B) 30 µg HeLa whole cell lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a BRE polyclonal antibody (Product # PA5-22154) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BRE using A) 30 µg Neuro2A whole cell lysate (B) 30 µg GL261 whole cell lysate and C) 30 µg C8D30 whole cell lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a BRE polyclonal antibody (Product # PA5-22154) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BRE using A) 30 µg PC-12 whole cell lysate and B) 30 µg Rat2 whole cell lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a BRE polyclonal antibody (Product # PA5-22154) at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of BRE in paraformaldehyde-fixed HeLa cells using a BRE polyclonal antibody (Product # PA5-22154) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of BRE in the nucleus of paraffin-embedded Cal27 xenograft using a BRE polyclonal antibody (Product # PA5-22154) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of BRE in the nucleus and cytosol of paraffin-embedded mouse pancreas tissue using a BRE polyclonal antibody (Product # PA5-22154) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of BRE in the nucleus and cytosol of paraffin-embedded mouse colon tissue using a BRE polyclonal antibody (Product # PA5-22154) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of BRE in the nucleus and cytosol of paraffin-embedded rat middle brain tissue using a BRE polyclonal antibody (Product # PA5-22154) at a dilution of 1:500.