Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [4]
- Immunohistochemistry [5]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-32270 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- hnRNP C1/C2 Recombinant Rabbit Monoclonal Antibody (SN0652)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
- Reactivity
- Human, Mouse, Rat, Zebrafish
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- SN0652
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of hnRNP C1/C2 in different lysates using a Monoclonal antibody (Product #MA5-32270) at a dilution of 1:1,000. Positive control: Lane 1: Hela, Lane 2: MCF-7, Lane 3: HepG2.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of hnRNP C1/C2 in different lysates using a Monoclonal antibody (Product #MA5-32270) at a dilution of 1:1,000. Positive control: Lane 1: Hela, Lane 2: MCF-7, Lane 3: HepG2.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-hnRNP C1/C2 Recombinant Rabbit Monoclonal Antibody (SN0652) (Product # MA5-32270) and a 40 kDa band corresponding to HNRNPC was observed across all cell lines tested. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), MCF7 (Lane 2), K-562 (Lane 3), Jurkat (Lane 4), MOLT-4 (Lane 5), HEK-293 (Lane 6), HeLa (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:10000 dilution) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of HNRNPC was achieved by transfecting HeLa with HNRNPC specific siRNAs (Silencer® select Product # S6719, S6721). Western blot analysis (Fig. a) was performed using whole cell extracts from the HNRNPC knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with hnRNP C1/C2 Recombinant Rabbit Monoclonal Antibody (SN0652) (Product # MA5-32270, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:10000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to HNRNPC.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of hnRNP C1/C2 in Hela cells using a hnRNP C1/C2 Monoclonal antibody (Product # MA5-32270) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of hnRNP C1/C2 in MCF-7 cells using a hnRNP C1/C2 Monoclonal antibody (Product # MA5-32270) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of hnRNP C1/C2 in B16-F1 cells using a hnRNP C1/C2 Monoclonal antibody (Product # MA5-32270) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HNRNPC was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with hnRNP C1/C2 Recombinant Rabbit Monoclonal Antibody (SN0652) (Product # MA5-32270) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with Hoechst 33342 (Product # H1399). F-actin (Panel c: Red) was stained with Alexa Fluor™ Plus 647 Phalloidin (Product # A30107, 1:2000 dilution). Panel d represents the merged image showing nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 40X magnification in CellInsight CX7 LZR High-Content Screening (HCS) Platform (Product # CX7A1110LZR) and externally deconvoluted (D.Sage et al. / Methods 115 (2017) 28–41).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of hnRNP C1/C2 of paraffin-embedded Human breast carcinoma tissue using a hnRNP-C1-C2 Monoclonal antibody (Product #MA5-32270). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of hnRNP C1/C2 of paraffin-embedded Human kidney tissue using a hnRNP-C1-C2 Monoclonal antibody (Product #MA5-32270). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of hnRNP C1/C2 of paraffin-embedded Mouse brain tissue using a hnRNP-C1-C2 Monoclonal antibody (Product #MA5-32270). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of hnRNP C1/C2 of paraffin-embedded Mouse skin tissue using a hnRNP-C1-C2 Monoclonal antibody (Product #MA5-32270). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of hnRNP C1/C2 of paraffin-embedded Mouse placenta tissue using a hnRNP-C1-C2 Monoclonal antibody (Product #MA5-32270). Counter stained with hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometric analysis of hnRNP C1/C2 in Hela cells using a hnRNP C1/C2 Monoclonal Antibody (Product # MA5-32270) at a dilution of 1:50, as seen in red compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.