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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 16-0519-81 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD51/CD61 (Integrin alpha v beta 3) Monoclonal Antibody (23C6), Functional Grade, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The 23C6 monoclonal antibody reacts with the human CD51/CD61 dimer, also known as the integrin alphav/beta3. CD51, an ~120 kDa surface molecule can also non-covalently associate with other beta subunits of the integrin family including beta1 (CD29), beta5 and beta6 to form receptors for extracellular matrix components. Heterodimers of CD51/CD61 are expressed by melanoma cells, endothelial cells and osteoclasts and at very low levels by platelets. The CD51/CD61 complex mediates adhesion to fibrinogen, fibronectin, vitronectin and thrombospondin.
- Antibody clone number
- 23C6
- Concentration
- 1 mg/mL
Submitted references High Density Display of an Anti-Angiogenic Peptide on Micelle Surfaces Enhances Their Inhibition of αvβ3 Integrin-Mediated Neovascularization In Vitro.
Pathological lymphangiogenesis is modulated by galectin-8-dependent crosstalk between podoplanin and integrin-associated VEGFR-3.
In vitro model of metastasis to bone marrow mediates prostate cancer castration resistant growth through paracrine and extracellular matrix factors.
Nagaraj R, Stack T, Yi S, Mathew B, Shull KR, Scott EA, Mathew MT, Bijukumar DR
Nanomaterials (Basel, Switzerland) 2020 Mar 22;10(3)
Nanomaterials (Basel, Switzerland) 2020 Mar 22;10(3)
Pathological lymphangiogenesis is modulated by galectin-8-dependent crosstalk between podoplanin and integrin-associated VEGFR-3.
Chen WS, Cao Z, Sugaya S, Lopez MJ, Sendra VG, Laver N, Leffler H, Nilsson UJ, Fu J, Song J, Xia L, Hamrah P, Panjwani N
Nature communications 2016 Apr 12;7:11302
Nature communications 2016 Apr 12;7:11302
In vitro model of metastasis to bone marrow mediates prostate cancer castration resistant growth through paracrine and extracellular matrix factors.
Lescarbeau RM, Seib FP, Prewitz M, Werner C, Kaplan DL
PloS one 2012;7(8):e40372
PloS one 2012;7(8):e40372
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of platelets with 0.5 µg of Mouse IgG1 kappa Isotype Control Purified (Product # 14-4714-82) (open histogram), or 0.5 µg of Anti-Human CD51/CD61 (Integrin alpha V beta 3) Purified followed by Anti-Mouse IgG FITC (Product # 11-4011-85) (filled histogram).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 Expression of alphavbeta3 by HUVECs under different conditions ( a ) Flow cytometry analysis demonstrated significant expression of integrin alphavbeta3 in overnight starved cells. (i) Side scatter (SSC) vs. forward scatter (FSC) plot used to gate live cells and resulting (ii) histograms from which alphavbeta3 expression was (iii) quantified for different culture conditions. Colors in (ii) match the conditions shown in the x-axis of (iii). Values were expressed as mean +- SD, n = 3. p < 0.005 was considered significant. ( b ) Immunostaining was performed by (i) staining HUVECs with anti-human CD51/CD61 antibody to detect integrin alphavbeta3 (green). Nuclei were stained by DAPI (blue). Integrin alphavbeta3 expression was highest under overnight starved conditions when compared to control and 2 h starvation. (ii) Quantification of the images in (i) obtained using Image J. Value was expressed as mean +- SD, n = 3. p < 0.05 was considered significant.
