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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- NBP1-31230 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-31230, RRID:AB_2216601
- Product name
- Rabbit Polyclonal WISP-1/CCN4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: WISP-1/CCN4 Antibody [NBP1-31230] - MCF7 lysate (25 ug loaded), stained with WISP1 antibody diluted at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: WISP-1/CCN4 Antibody [NBP1-31230] - A:A431 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: WISP-1/CCN4 Antibody [NBP1-31230] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with WISP1 antibody [N3C1], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: WISP-1/CCN4 Antibody [NBP1-31230] - Methanol-fixed A431, using WISP1 antibody at 1:200 dilution.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: WISP-1/CCN4 Antibody [NBP1-31230] - Paraffin-embedded HT29 xenograft, using antibody at 1:100 dilution.
