NB100-77339

antibody from Novus Biologicals

Targeting: CCN4 WISP-1, WISP1, WISP1-OT1, WISP1-UT1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Antibody Data

Product number

NB100-77339

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-77339, RRID:AB_1085989

Product name

Goat Polyclonal WISP-1/CCN4 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. This antibody is expected to recognize both reported isoforms (NP_003873.1

Reactivity

Human

Host

Goat

Antigen sequence

ESYPDFSEIAN


Isotype

IgG

Vial size

0.1 mg

Concentration

0.5 mg/ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

References

Submitted references

Wilms tumor suppressor WTX negatively regulates WNT/beta-catenin signaling.

Major MB, Camp ND, Berndt JD, Yi X, Goldenberg SJ, Hubbert C, Biechele TL, Gingras AC, Zheng N, Maccoss MJ, Angers S, Moon RT
Science (New York, N.Y.) 2007 May 18;316(5827):1043-6

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: WISP-1/CCN4 Antibody [NB100-77339] - Analysis using NB100-77339 at 0.1ug/ml in HEK293 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: WISP-1/CCN4 Antibody [NB100-77339] - Analysis of HEK293 overexpressing WISP1 with C-terminal tag (DYKDDDDK) and probed with anti-DYKDDDDK in the left panel and with (mock transfection in first and last lanes).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: WISP-1/CCN4 Antibody [NB100-77339] - HEK293 overexpressing WISP1 (RC214390) with C-terminal tag (DYKDDDDK) and probed with anti-DYKDDDDK in the left panel and with EB08178 in the right panel (mock transfection in first lane in each panel).