Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-47124 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Galectin 7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In sandwich ELISAs, approximately 50% cross-reactivity with recombinant mouse (rm) Galectin-7 is observed and less than 0.5% cross-reactivity with recombinant human (rh) Galectin-1, rmGalectin-3, rhGalectin-4, and rhGalectin-8 is observed. Reconstitute at 0.2 mg/mL in sterile PBS.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of HEK001 human epidermal keratinocyte cell line, loaded at 0.2 mg/mL. A specific band was detected for Galectin-7 at approximately 21 kDa (as indicated) using 10 µg/mL of Goat Anti-human Galectin-7 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47124)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of human skin tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-human Galectin-7 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47124) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for Galectin-7 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions. Western blot analysis from lysates of HEK001 human epidermal keratinocyte cell line, loaded at 0.2 mg/mL. A specific band was detected for Galectin-7 at approximately 21 kDa (as indicated) using 10 µg/mL of Goat Anti-human Galectin-7 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47124) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody. This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Galectin 7 in human skin tissue. Samples were incubated in Galectin 7 polyclonal antibody (Product # PA5-47124) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. A specific band was detected for Galectin‚7 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Galectin 7 in 0.2 mg/mL lysates of HEK001 human epidermal keratinocyte cell line. Samples were incubated in Galectin 7 polyclonal antibody (Product # PA5-47124) using a dilution of 10 µg/mL followed by HRP-conjugated Anti-Goat IgG at a dilution of 0.0763888888888889. A specific band was detected for Galectin‚7 at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
