PA5-47003

antibody from Invitrogen Antibodies

Targeting: IL23A IL-23, IL-23A, IL23P19, P19, SGRF

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-47003

Provider

Invitrogen Antibodies

Product name

IL-12/IL-23 p40 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

In direct ELISAs, no cross-reactivity with recombinant human IL-23 p19 and recombinant mouse IL-23 p19 was observed.

Concentration

0.2 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis from lysates of Hs 294T human melanoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-human IL-12/IL-23 p40 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47003) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for IL-12/IL-23 p40 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of IL-12/IL-23 p40 in Hs 294T human melanoma cell line. Samples were incubated in IL-12/IL-23 p40 polyclonal antibody (Product # PA5-47003) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. A specific band was detected for IL‚12/IL‚23 p40 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Neutralization antibody testing demonstrates the specificity of an antibody through a correlation between antibody binding and the activity of the target. Neutralization of IL-12/IL-23 p40 is shown by the decrease in mean OD (measure of IL-17 secretion) with increasing concentrations of IL-12/IL-23 p40 Polyclonal Antibody (PA5-47003).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Neutralization of IL-12/IL-23 p40 in mouse splenocytes. Samples were incubated in IL-12/IL-23 p40 polyclonal antibody (Product # PA5-47003). Recombinant Human IL‚23 induces IL-17 secretion in the mouse splenocytes in a dose‚dependent manner (orange line), as measured by Mouse IL-17 Quantikine kit. IL-17 Secretion elicited by Recombinant Human IL‚23 (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti‚Human IL‚12/IL‚23 p40 Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 0.004-0.02 μg/mL.