14-7127-81

antibody from Invitrogen Antibodies

Targeting: IL23A IL-23, IL-23A, IL23P19, P19, SGRF

ELISA (Recommended by provider)

Antibody Data

Product number

14-7127-81

Provider

Invitrogen Antibodies

Product name

IL-12/IL-23 p40 Monoclonal Antibody (C8.3), eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Description: The C8.3 antibody reacts with the p40 monomer of human IL-12 and IL-23; the antibody preferentially binds to monomeric p40. Interleukin-12 (IL-12) is a heterodimeric 70 kD (p70) cytokine composed of two covalently linked, glycosylated chains, 40kD (p40) and 35-kD (p35). IL-12 is mainly produced by monocytes, macrophages, and dendritic cells in response to bacterial products such as lipopolysaccharides (LPS), to intracellular pathogens or upon interaction with activated T cells. IL-12 was originally discovered because of its ability to induce interferon-gamma production, cell proliferation, and cytotoxicity mediated by natural killer cells and T cells. It is now established that IL-12 also plays a key role in the development of Th1 responses, leading to IFN-gamma and IL-2 production. These cytokines can in turn promote T-cell responses and macrophage activation. The p40 and p35 subunits by themselves have no IL-12 bioactivity, though the p40 homodimer has been shown to bind the IL-12 receptor and to be an antagonist of IL-12 p70. Free p40 is typically secreted in vast excess of IL-12 p70 by cells co-expressing both the p35 and p40 subunits. The p40 subunit of IL-12 has been shown to have extensive amino acid sequence homology to the extracellular domain of the IL-6 receptor, while the p35 subunit shows some homology to IL-6 and G-CSF. Applications Reported: The C8.3 antibody has been reported for use in ELISA. Applications Tested: The IL-12/IL-23 p40 monomer ELISA using clones C8.3 and C8.6 preferentially measures p40 monomer, exhibiting less than 1.0% cross reactivity with IL-12 (p35p40) and IL-23 (p19p40). For this assay, the (p40 monomer-specific) purified C8.3 antibody (14-7127) is used as the capture antibody in combination with the biotinylated C8.6 antibody (13-7129) for detection and recombinant human IL-12/IL-23 p40 (39-8128) as the standard. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 10,000 pg/mL - 80.0 pg/mL should be included in each ELISA plate. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/mL. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

C8.3

Vial size

50 µg

Concentration

0.5 mg/mL

Storage

4° C