Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 12-7129-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL-12/IL-23 p40 Monoclonal Antibody (C8.6), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The C8.6 antibody reacts with the p40 subunit of human IL-12 p70 and human IL-23. IL-12 is a 70 kDa heterodimeric cytokine composed of two covalently linked chains, p40 and p35, and secreted primarily by activated monocytes, macrophages, and dendritic cells. IL-23 is a biologically-related, heterodimeric cytokine composed of disulfide-linked p40 and p19 subunits. Applications Reported:The C8.6 antibody has been reported for use in intracellular flow cytometric analysis. Applications Tested: Has been tested by intracellular flow cytometric analysis. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. The pre-titrated test size contains BSA and/or gelatin for protein stabilization. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Antibody clone number
- C8.6
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Cell membrane-anchored and tumor-targeted IL-12 (attIL12)-T cell therapy for eliminating large and heterogeneous solid tumors.
Monocyte subsets in schistosomiasis patients with periportal fibrosis.
Hepatic enrichment and activation of myeloid dendritic cells during chronic hepatitis C virus infection.
Direct and indirect impairment of human dendritic cell function by virulent Francisella tularensis Schu S4.
Hu J, Yang Q, Zhang W, Du H, Chen Y, Zhao Q, Dao L, Xia X, Natalie Wall F, Zhang Z, Mahadeo K, Gorlick R, Kopetz S, Dotti G, Li S
Journal for immunotherapy of cancer 2022 Jan;10(1)
Journal for immunotherapy of cancer 2022 Jan;10(1)
Monocyte subsets in schistosomiasis patients with periportal fibrosis.
Fernandes JS, Araujo MI, Lopes DM, de Souza Rda P, Carvalho EM, Cardoso LS
Mediators of inflammation 2014;2014:703653
Mediators of inflammation 2014;2014:703653
Hepatic enrichment and activation of myeloid dendritic cells during chronic hepatitis C virus infection.
Velazquez VM, Hon H, Ibegbu C, Knechtle SJ, Kirk AD, Grakoui A
Hepatology (Baltimore, Md.) 2012 Dec;56(6):2071-81
Hepatology (Baltimore, Md.) 2012 Dec;56(6):2071-81
Direct and indirect impairment of human dendritic cell function by virulent Francisella tularensis Schu S4.
Chase JC, Celli J, Bosio CM
Infection and immunity 2009 Jan;77(1):180-95
Infection and immunity 2009 Jan;77(1):180-95
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Intracellular staining of unstimulated (left) and 2-hour IFN gamma/overnight LPS-stimulated (right) human peripheral blood cells with staining buffer (autofluorescence) (open histogram) or Anti-Human IL-12/IL-23 p40 PE (filled histogram). Cells in the monocyte gate were used for analysis.
- Conjugate
- Yellow dye