Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 12-7235-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL-12/IL-23 p40 Monoclonal Antibody (eBioHP40 (HP40, HP-40)), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The eBioHP40 antibody reacts with the p40 subunit of human IL-12 p70, and human IL-23. IL-12 is a 70 kDa heterodimeric cytokine composed of two covalently linked chains, p40 and p35, and is secreted primarily by activated monocytes, macrophages, and dendritic cells. IL-23 is a biologically-related, heterodimeric cytokine composed of disulfide-linked p40 and p19 subunits. The eBioHP40 antibody has slightly lower background staining than C8.6 and recognizes a different epitope. Applications Reported: The C8.6 antibody has been reported for use in intracellular flow cytometric analysis. Applications Tested: This eBioHP40 (HP40, HP-40) antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Antibody clone number
- eBioHP40 (HP40, HP-40)
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Chloroquine promotes IL-17 production by CD4+ T cells via p38-dependent IL-23 release by monocyte-derived Langerhans-like cells.
Said A, Bock S, Lajqi T, Müller G, Weindl G
Journal of immunology (Baltimore, Md. : 1950) 2014 Dec 15;193(12):6135-43
Journal of immunology (Baltimore, Md. : 1950) 2014 Dec 15;193(12):6135-43
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Intracellular staining of unstimulated (left) or IFN gamma (Product # 14-8139), LPS, and monensin stimulated (right) normal human peripheral blood cells with Anti-Human CD14 FITC (Product # 11-0149-42) and Anti-Human IL-12/IL-23 p40 PE. Cells in the monocyte gate were used for analysis.
- Conjugate
- Yellow dye