Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 48-7129-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL-12/IL-23 p40 Monoclonal Antibody (C8.6), eFluor™ 450, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The C8.6 antibody reacts with the p40 subunit of human IL-12 p70 and human IL-23. IL-12 is a 70 kDa heterodimeric cytokine composed of two covalently linked chains, p40 and p35, and secreted primarily by activated monocytes, macrophages, and dendritic cells. IL-23 is a biologically-related, heterodimeric cytokine composed of disulfide-linked p40 and p19 subunits. Applications Reported: This C8.6 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This C8.6 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- C8.6
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Intracellular staining of IFN gamma/LPS-stimulated normal human peripheral blood cells with Mouse IgG1 K Isotype Control eFluor® 450 (Product # 48-4714-82) (blue histogram) or Anti-Human IL-12/ IL-23 p40 eFluor® 450 (purple histogram). Total viable cells were used for analysis.